TY - JOUR
T1 - Activin A-induced apoptosis is suppressed by BCL-2
AU - Koseki, Takeyoshi
AU - Yamato, Kenji
AU - Krajewski, Stanislaw
AU - Reed, John C.
AU - Tsujimoto, Yoshihide
AU - Nishihara, Tatsuji
N1 - Funding Information:
Acknowledgements: This work is supported in part by National Institute Post Doctoral Fellowship of the Research Development Corporation of Japan and Grants-in aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1995/12/4
Y1 - 1995/12/4
N2 - Activin A, a member of TGFβ superfamily has various activities including induction of apoptosis in mammalian cells. However, it remains unknown how activin A induces cell death. To clarify this, we investigated the expression of BCL-2 and BAX, and the effect of BCL-2 overexpression on activin A-induced apoptosis in B cell hybridoma cell lines. The activin A-sensitive cell lines expressed BAX but not BCL-2 and that activin A did not increase BAX levels. Overexpression of human BCL-2 suppressed activin A-induced apoptosis in these cells. Thus, activin A has been shown to induce apoptosis by a BCL-2-inhibitable mechanism without activating BAX.
AB - Activin A, a member of TGFβ superfamily has various activities including induction of apoptosis in mammalian cells. However, it remains unknown how activin A induces cell death. To clarify this, we investigated the expression of BCL-2 and BAX, and the effect of BCL-2 overexpression on activin A-induced apoptosis in B cell hybridoma cell lines. The activin A-sensitive cell lines expressed BAX but not BCL-2 and that activin A did not increase BAX levels. Overexpression of human BCL-2 suppressed activin A-induced apoptosis in these cells. Thus, activin A has been shown to induce apoptosis by a BCL-2-inhibitable mechanism without activating BAX.
KW - Activin A
KW - Apoptosis
KW - BAX
KW - BCL-2
KW - Programmed cell death
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U2 - 10.1016/0014-5793(95)01290-7
DO - 10.1016/0014-5793(95)01290-7
M3 - Article
C2 - 7498552
AN - SCOPUS:0028799285
SN - 0014-5793
VL - 376
SP - 247
EP - 250
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -