Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish)

Tomohiko Taguchi; Ken Kitajima; Sadako Inoue; Yasuo Inoue; Ji Mao Yang; Harry Schachter; Inka Brockhausen

doi:10.1006/bbrc.1996.6013

Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish)

Tomohiko Taguchi, Ken Kitajima, Sadako Inoue, Yasuo Inoue, Ji Mao Yang, Harry Schachter, Inka Brockhausen

LIF - Integrative Life Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

UDP-GlcNAc:GlcNAcβ1→(GlcNAcβ1→2)Manα1-R[GlcNAc to Man]β1→4N-acetylglucosaminyltransferase VI (GnT VI) activity was shown to be present in crude homogenates of Medaka fish (Oryzias latipes) ovaries using UDP-[¹⁴C]GlcNAc and synthetic GlcNAcβ1→6 (GlcNAcβ1→2)Manα1→6Glcβ1→octyl as substrates. Characterization of this activity showed a pH optimum at about pH 7.0 and an absolute requirement for divalent cations. The optimum concentration of Mn²⁺ was at about 25 mM. This finding is the first report on GnT VI activity in fish; the enzyme has previously been described only in avian tissues.

Original language	English
Pages (from-to)	533-536
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	230
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1996.6013
Publication status	Published - 1997 Jan 23

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10.1006/bbrc.1996.6013

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Taguchi, T., Kitajima, K., Inoue, S., Inoue, Y., Yang, J. M., Schachter, H., & Brockhausen, I. (1997). Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish). Biochemical and Biophysical Research Communications, 230(3), 533-536. https://doi.org/10.1006/bbrc.1996.6013

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title = "Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish)",

abstract = "UDP-GlcNAc:GlcNAcβ1→(GlcNAcβ1→2)Manα1-R[GlcNAc to Man]β1→4N-acetylglucosaminyltransferase VI (GnT VI) activity was shown to be present in crude homogenates of Medaka fish (Oryzias latipes) ovaries using UDP-[14C]GlcNAc and synthetic GlcNAcβ1→6 (GlcNAcβ1→2)Manα1→6Glcβ1→octyl as substrates. Characterization of this activity showed a pH optimum at about pH 7.0 and an absolute requirement for divalent cations. The optimum concentration of Mn2+ was at about 25 mM. This finding is the first report on GnT VI activity in fish; the enzyme has previously been described only in avian tissues.",

author = "Tomohiko Taguchi and Ken Kitajima and Sadako Inoue and Yasuo Inoue and Yang, {Ji Mao} and Harry Schachter and Inka Brockhausen",

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doi = "10.1006/bbrc.1996.6013",

language = "English",

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Taguchi, T, Kitajima, K, Inoue, S, Inoue, Y, Yang, JM, Schachter, H & Brockhausen, I 1997, 'Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish)', Biochemical and Biophysical Research Communications, vol. 230, no. 3, pp. 533-536. https://doi.org/10.1006/bbrc.1996.6013

Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish). / Taguchi, Tomohiko; Kitajima, Ken; Inoue, Sadako et al.
In: Biochemical and Biophysical Research Communications, Vol. 230, No. 3, 23.01.1997, p. 533-536.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Taguchi, Tomohiko

AU - Kitajima, Ken

AU - Inoue, Sadako

AU - Inoue, Yasuo

AU - Yang, Ji Mao

AU - Schachter, Harry

AU - Brockhausen, Inka

PY - 1997/1/23

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Activity of UDP-GlcNAc:GLcNacβ1→6(GlcNacβ1→2)Manα1→R[GlcNAc to Man]β1→4AN-Acetyglucosaminyltransferase VI (GNT VI) from the ovaries of Oryzias latipes (Medaka fish)

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