Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage

Zhi Hua Chen; Yasukazu Yoshida; Yoshiro Saito; Etsuo Niki

doi:10.1016/j.neulet.2005.04.022

Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage

Zhi Hua Chen, Yasukazu Yoshida, Yoshiro Saito, Etsuo Niki

PHA - Life and Pharmaceutical Science

National Institute of Advanced Industrial Science and Technology

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The adaptive responses to H₂O₂ and the resulting protective effect against oxidative stress have been investigated using PC12 cells. Pretreatment of sublethal doses of H₂O₂ significantly protected PC12 cells against the cytotoxicity induced by lethal H₂O₂. The endogenous antioxidant defense systems, catalase and glutathione peroxidase, were enhanced quickly by the pretreatment of low doses of H₂O₂. This pretreatment also exerted protective effect against the oxidative insults induced by 6-hydroxydopamine and paraquat, but not against alkyl peroxyl radicals. Our results, taken together, suggest that the stimulation by low dose of H₂O₂ enriches the cellular antioxidant defense systems, thereby enhancing cell tolerance against the forthcoming oxidative insults induced by H₂O₂ and related hydroxyl radicals.

Original language	English
Pages (from-to)	256-259
Number of pages	4
Journal	Neuroscience Letters
Volume	383
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2005.04.022
Publication status	Published - 2005 Aug 5

Keywords

Adaptation
Catalase
Glutathione peroxidase
Hydrogen peroxide

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10.1016/j.neulet.2005.04.022

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abstract = "The adaptive responses to H2O2 and the resulting protective effect against oxidative stress have been investigated using PC12 cells. Pretreatment of sublethal doses of H2O2 significantly protected PC12 cells against the cytotoxicity induced by lethal H2O2. The endogenous antioxidant defense systems, catalase and glutathione peroxidase, were enhanced quickly by the pretreatment of low doses of H2O2. This pretreatment also exerted protective effect against the oxidative insults induced by 6-hydroxydopamine and paraquat, but not against alkyl peroxyl radicals. Our results, taken together, suggest that the stimulation by low dose of H2O2 enriches the cellular antioxidant defense systems, thereby enhancing cell tolerance against the forthcoming oxidative insults induced by H2O2 and related hydroxyl radicals.",

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T1 - Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage

AU - Chen, Zhi Hua

AU - Yoshida, Yasukazu

AU - Saito, Yoshiro

AU - Niki, Etsuo

PY - 2005/8/5

Y1 - 2005/8/5

N2 - The adaptive responses to H2O2 and the resulting protective effect against oxidative stress have been investigated using PC12 cells. Pretreatment of sublethal doses of H2O2 significantly protected PC12 cells against the cytotoxicity induced by lethal H2O2. The endogenous antioxidant defense systems, catalase and glutathione peroxidase, were enhanced quickly by the pretreatment of low doses of H2O2. This pretreatment also exerted protective effect against the oxidative insults induced by 6-hydroxydopamine and paraquat, but not against alkyl peroxyl radicals. Our results, taken together, suggest that the stimulation by low dose of H2O2 enriches the cellular antioxidant defense systems, thereby enhancing cell tolerance against the forthcoming oxidative insults induced by H2O2 and related hydroxyl radicals.

AB - The adaptive responses to H2O2 and the resulting protective effect against oxidative stress have been investigated using PC12 cells. Pretreatment of sublethal doses of H2O2 significantly protected PC12 cells against the cytotoxicity induced by lethal H2O2. The endogenous antioxidant defense systems, catalase and glutathione peroxidase, were enhanced quickly by the pretreatment of low doses of H2O2. This pretreatment also exerted protective effect against the oxidative insults induced by 6-hydroxydopamine and paraquat, but not against alkyl peroxyl radicals. Our results, taken together, suggest that the stimulation by low dose of H2O2 enriches the cellular antioxidant defense systems, thereby enhancing cell tolerance against the forthcoming oxidative insults induced by H2O2 and related hydroxyl radicals.

KW - Adaptation

KW - Catalase

KW - Glutathione peroxidase

KW - Hydrogen peroxide

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Adaptation to hydrogen peroxide enhances PC12 cell tolerance against oxidative damage

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Keywords

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