Adhesion to cells of enzyme conjugated phospholipid polymer nanoparticles

Tomomi Ito, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara

Research output: Contribution to journalArticlepeer-review


We investigated the bioconjugation of proteins on polymer nanoparticles covered with bio-inert phosphorylcholine groups. Poly[2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl polyethyleneglycol methacrylate] was used as an emulsifier and a surface modifier to prepare the poly (L-lactic acid) nanoparticles by a solvent evaporation technique. The diameter and surface potential of the nanoparticles were approximately 260 nm and -5 mV, respectively. We considered that the polymer chain that transformed the conformation by change in pH may be used as a method of controlling the bio-reaction. The enzyme activity in a different pH under the coexistence of poly (glutamic acid) (PGA) and the enzyme was measured. Therefore, the enzyme activity increased in pH 6 in PGA/enzyme mixture system compared with that in pH 7 while the activity was constant in the enzyme single conjugation regardless of the pH change.

Original languageEnglish
Pages (from-to)785-788
Number of pages4
JournalKey Engineering Materials
Publication statusPublished - 2007


  • Bioconjugation
  • Cell-targeting diagnosis
  • Nanoparticles
  • Phospholipid polymer


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