Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells

Yin Li; Yan Zhang; Shigeki Shibahara; Kazuhiro Takahashi

doi:10.1016/j.bbrc.2006.09.089

Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells

Yin Li, Yan Zhang, Shigeki Shibahara, Kazuhiro Takahashi

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Expression of adrenomedullin (AM), a potent vasodilator peptide, was studied during adipocyte differentiation of human mesenchymal stem cells (hMSCs). Immunoreactive AM levels in the medium were increased at day 4 and 8 of the adipocyte differentiation. Northern blot analysis showed increased expression of AM mRNA in hMSCs-derived adipocytes at day 4, 8, 12, and 18. Transient transfection assay showed that the promoter activity was higher in hMSCs-derived adipocytes than in hMSCs, when cells were transfected with plasmids containing a cis-acting region (-70/-29) of the human AM gene. Electrophoretic mobility shift assay showed that specific bands bound to the region (-70/-29) in hMSCs-derived adipocytes but not in hMSCs, and were abolished by the stimulatory protein 1 (Sp1) antibody. The present study has shown that AM expression is up-regulated during adipocyte differentiation of hMSCs probably via the interaction between Sp1 or Sp1-related factor(s) and the AM promoter region (-70/-29).

Original language	English
Pages (from-to)	616-622
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	350
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2006.09.089
Publication status	Published - 2006 Nov 24

Keywords

Adipocytes
Adrenomedullin
Differentiation
Expression
Gene

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.09.089

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title = "Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells",

abstract = "Expression of adrenomedullin (AM), a potent vasodilator peptide, was studied during adipocyte differentiation of human mesenchymal stem cells (hMSCs). Immunoreactive AM levels in the medium were increased at day 4 and 8 of the adipocyte differentiation. Northern blot analysis showed increased expression of AM mRNA in hMSCs-derived adipocytes at day 4, 8, 12, and 18. Transient transfection assay showed that the promoter activity was higher in hMSCs-derived adipocytes than in hMSCs, when cells were transfected with plasmids containing a cis-acting region (-70/-29) of the human AM gene. Electrophoretic mobility shift assay showed that specific bands bound to the region (-70/-29) in hMSCs-derived adipocytes but not in hMSCs, and were abolished by the stimulatory protein 1 (Sp1) antibody. The present study has shown that AM expression is up-regulated during adipocyte differentiation of hMSCs probably via the interaction between Sp1 or Sp1-related factor(s) and the AM promoter region (-70/-29).",

keywords = "Adipocytes, Adrenomedullin, Differentiation, Expression, Gene",

author = "Yin Li and Yan Zhang and Shigeki Shibahara and Kazuhiro Takahashi",

note = "Funding Information: This work was supported in part by the 21st COE programs {\textquoteleft}Center for Innovative Therapeutic Development towards the Conquest of Signal Transduction Diseases{\textquoteright} (to Y.L., Y.Z., S.S.) and {\textquoteleft}Comprehensive Research and Education Center for Drug Development and Clinical Evaluation (CRESCENDO){\textquoteright} (to K.T.), a Grant-in-Aid or Scientific Research (C) from Japan Society for the Promotion of Science (2006–2007), and by a Research Grant from the Takeda Research Foundation (2005).",

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doi = "10.1016/j.bbrc.2006.09.089",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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AU - Li, Yin

AU - Zhang, Yan

AU - Shibahara, Shigeki

AU - Takahashi, Kazuhiro

N1 - Funding Information: This work was supported in part by the 21st COE programs ‘Center for Innovative Therapeutic Development towards the Conquest of Signal Transduction Diseases’ (to Y.L., Y.Z., S.S.) and ‘Comprehensive Research and Education Center for Drug Development and Clinical Evaluation (CRESCENDO)’ (to K.T.), a Grant-in-Aid or Scientific Research (C) from Japan Society for the Promotion of Science (2006–2007), and by a Research Grant from the Takeda Research Foundation (2005).

PY - 2006/11/24

Y1 - 2006/11/24

N2 - Expression of adrenomedullin (AM), a potent vasodilator peptide, was studied during adipocyte differentiation of human mesenchymal stem cells (hMSCs). Immunoreactive AM levels in the medium were increased at day 4 and 8 of the adipocyte differentiation. Northern blot analysis showed increased expression of AM mRNA in hMSCs-derived adipocytes at day 4, 8, 12, and 18. Transient transfection assay showed that the promoter activity was higher in hMSCs-derived adipocytes than in hMSCs, when cells were transfected with plasmids containing a cis-acting region (-70/-29) of the human AM gene. Electrophoretic mobility shift assay showed that specific bands bound to the region (-70/-29) in hMSCs-derived adipocytes but not in hMSCs, and were abolished by the stimulatory protein 1 (Sp1) antibody. The present study has shown that AM expression is up-regulated during adipocyte differentiation of hMSCs probably via the interaction between Sp1 or Sp1-related factor(s) and the AM promoter region (-70/-29).

AB - Expression of adrenomedullin (AM), a potent vasodilator peptide, was studied during adipocyte differentiation of human mesenchymal stem cells (hMSCs). Immunoreactive AM levels in the medium were increased at day 4 and 8 of the adipocyte differentiation. Northern blot analysis showed increased expression of AM mRNA in hMSCs-derived adipocytes at day 4, 8, 12, and 18. Transient transfection assay showed that the promoter activity was higher in hMSCs-derived adipocytes than in hMSCs, when cells were transfected with plasmids containing a cis-acting region (-70/-29) of the human AM gene. Electrophoretic mobility shift assay showed that specific bands bound to the region (-70/-29) in hMSCs-derived adipocytes but not in hMSCs, and were abolished by the stimulatory protein 1 (Sp1) antibody. The present study has shown that AM expression is up-regulated during adipocyte differentiation of hMSCs probably via the interaction between Sp1 or Sp1-related factor(s) and the AM promoter region (-70/-29).

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KW - Expression

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Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells

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