TY - JOUR
T1 - Adsorption behavior of propranolol on negatively-charged liposomes and its influence on membrane fluidity and polarity
AU - Ishigami, Takaaki
AU - Chern, Michael Sun
AU - Suga, Keishi
AU - Okamoto, Yukihiro
AU - Umakoshi, Hiroshi
N1 - Funding Information:
This research was supported by a Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for JSPS Fellows number 13J03878, the Funding Program for Next Generation World-Leading Researchers of the Council for Science and Technology Policy (CSTP) (GR066), JSPS Grant-in-Aid for Scientific Research A (26249116) and a JSPS Grant-in-Aid for Research Activity Start-up (25889039). One of the authors (Takaaki Ishigami) expresses his gratitude for the Japan Society for the Promotion of Science (JSPS) scholarships.
Publisher Copyright:
Copyright © 2017 American Scientific Publishers All rights reserved.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - The adsorption behaviors of R-propranolol (PPL) for the negatively-charged liposomes were studied focusing on the liposome membrane properties. In the case of the negatively-charged DMPA and DMPS liposomes, the adsorbed amount of PPL on the membrane surface was high, while little amount of PPL did on the neutral DMPC liposome. Using fluorescent probes, the membrane properties such as membrane fluidity and membrane polarity were investigated. The PPL adsorption on the negatively-charged liposomes induced an increase in membrane fluidity, and the membrane surface turned to be hydrated. Raman spectroscopic analysis revealed the decrease of packing density of DMPA and DMPS liposomes in the presence of PPL molecules. It is considered that the PPL molecules can adsorb on the membranes through not only electrostatic interaction but also other weak interactions such as hydrophobic interaction and hydrogen bond interaction. These findings also suggest the significance of the membrane properties, i.e., surface charge, membrane fluidity, and membrane polarity, in understanding the adsorption behaviors of chemicals on the self-assembled membranes.
AB - The adsorption behaviors of R-propranolol (PPL) for the negatively-charged liposomes were studied focusing on the liposome membrane properties. In the case of the negatively-charged DMPA and DMPS liposomes, the adsorbed amount of PPL on the membrane surface was high, while little amount of PPL did on the neutral DMPC liposome. Using fluorescent probes, the membrane properties such as membrane fluidity and membrane polarity were investigated. The PPL adsorption on the negatively-charged liposomes induced an increase in membrane fluidity, and the membrane surface turned to be hydrated. Raman spectroscopic analysis revealed the decrease of packing density of DMPA and DMPS liposomes in the presence of PPL molecules. It is considered that the PPL molecules can adsorb on the membranes through not only electrostatic interaction but also other weak interactions such as hydrophobic interaction and hydrogen bond interaction. These findings also suggest the significance of the membrane properties, i.e., surface charge, membrane fluidity, and membrane polarity, in understanding the adsorption behaviors of chemicals on the self-assembled membranes.
KW - Membrane fluidity
KW - Membrane polarity
KW - Negatively-charged liposome
KW - Propranolol
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U2 - 10.1166/jnn.2017.12823
DO - 10.1166/jnn.2017.12823
M3 - Article
AN - SCOPUS:85009976293
SN - 1533-4880
VL - 17
SP - 1721
EP - 1728
JO - Journal of Nanoscience and Nanotechnology
JF - Journal of Nanoscience and Nanotechnology
IS - 3
ER -