TY - JOUR
T1 - Advanced application of porcine intestinal epithelial cells for the selection of immunobiotics modulating toll-like receptor 3-mediated inflammation
AU - Hosoya, Shoichi
AU - Villena, Julio
AU - Chiba, Eriko
AU - Shimazu, Tomoyuki
AU - Suda, Yoshihito
AU - Aso, Hisashi
AU - Saito, Tadao
AU - Kitazawa, Haruki
N1 - Funding Information:
We sincerely thank Drs Shuji Ikegami and Hiroyuki Itoh (Division of Research and Development, Meiji Co.) for kindly providing the lactobacilli strains, and Drs Yasushi Kawai (Tohoku University), Masanori Tohno (National Institute of Livestock and Grassland Science) and Takeshi Shimosato (Shinshu University) for their helpful discussion, and also Ms Junko Nishimura for her technical support with the bacterial studies. This study was supported by a Grant-in-Aid for Scientific Research (B)(2) (No. 21380164), Challenging Exploratory Research (No. 20658061, 23658216) and JSPS fellows (No. 2109335) from the Japan Society for the Promotion of Science (JSPS) to Dr H. Kitazawa. Dr J. Villena was supported by JSPS (Postdoctoral Fellowship for Foreign Researchers, Program No. 21-09335).
PY - 2013/12
Y1 - 2013/12
N2 - Purpose: In this study, we aimed to characterize toll-like receptor (TLR)-3-mediated inflammatory immune response in porcine intestinal epithelial (PIE) cells and in PIE-immune cell co-cultures and, to evaluate if these in vitro systems are useful for selecting immunomodulatory lactic acid bacteria. Results: We demonstrated that these systems are valuable tools for the in vitro study of the inflammatory response triggered by TLR3 in intestinal epithelial cells (IECs) and of the interaction between IECs and immune cells. In addition, we showed that PIE cells could be used for the selection of immunobiotic lactobacilli strains with anti-inflammatory activities. We found that Lactobacillus casei MEP221114 is an immunobiotic candidate for modulation of TLR3-mediated inflammatory responses. Conclusion: The present study deepened our understanding of the mechanisms of immunobiotic action by demonstrating that the interaction between some lactobacilli strains and IECs can up-regulate the mRNA expression of TLR negative regulators and that this effect could help to regulate the production of inflammatory mediators during the generation of a TLR3-mediated immune response.
AB - Purpose: In this study, we aimed to characterize toll-like receptor (TLR)-3-mediated inflammatory immune response in porcine intestinal epithelial (PIE) cells and in PIE-immune cell co-cultures and, to evaluate if these in vitro systems are useful for selecting immunomodulatory lactic acid bacteria. Results: We demonstrated that these systems are valuable tools for the in vitro study of the inflammatory response triggered by TLR3 in intestinal epithelial cells (IECs) and of the interaction between IECs and immune cells. In addition, we showed that PIE cells could be used for the selection of immunobiotic lactobacilli strains with anti-inflammatory activities. We found that Lactobacillus casei MEP221114 is an immunobiotic candidate for modulation of TLR3-mediated inflammatory responses. Conclusion: The present study deepened our understanding of the mechanisms of immunobiotic action by demonstrating that the interaction between some lactobacilli strains and IECs can up-regulate the mRNA expression of TLR negative regulators and that this effect could help to regulate the production of inflammatory mediators during the generation of a TLR3-mediated immune response.
KW - Anti-inflammatory response
KW - Immunobiotic
KW - Porcine intestinal cepithelial cells
KW - Toll-like receptor 3
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U2 - 10.1016/j.jmii.2012.04.005
DO - 10.1016/j.jmii.2012.04.005
M3 - Article
C2 - 22727542
AN - SCOPUS:84890122446
SN - 1684-1182
VL - 46
SP - 474
EP - 481
JO - Journal of Microbiology, Immunology and Infection
JF - Journal of Microbiology, Immunology and Infection
IS - 6
ER -
