Advantages of zero-loss imaging in transmission electron microtomography

Yukihiro Nishikawa, Takeshi Kaneko, Hideo Nishioka, Toshio Nishi, Hiroshi Jinnai

Research output: Contribution to conferencePaperpeer-review


Transmission electron microtomography (TEMT) is a powerful technique to investigate the three-dimensional structures in nanometer scale, which is based on the transmission electron microscopy (TEM) and computerized tomography (CT). In this study, we demonstrate the advantage of zero-loss imaging based on electron energy loss spectroscopy (EELS) in the quantitative data acquisition for TEMT. Transmission of zero-loss image during tilting the specimen agreed with the calculated transmission under assumption of the increase of effective specimen thickness, which is necessity to obtain the quantitative reconstructions according to the principle of CT. On the other hand, the transmission of unfiltered images was almost constant during tilting. It is concluded that the zero-loss imaging is always required for TEMT.

Original languageEnglish
Number of pages2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sept 202005 Sept 22


Conference54th SPSJ Symposium on Macromolecules


  • 3d image analysis
  • Computer tomography
  • Electron energy loss spectroscopy
  • Transmission electron microscopy
  • Zero-loss imaging


Dive into the research topics of 'Advantages of zero-loss imaging in transmission electron microtomography'. Together they form a unique fingerprint.

Cite this