TY - JOUR
T1 - Aerial mycelium formation in rare thermophilic Actinobacteria on media solidified with agar and gellan gum
AU - Sari, D. C.A.F.
AU - Ningsih, F.
AU - Yokota, A.
AU - Yabe, S.
AU - Sjamsuridzal, W.
AU - Oetari, A.
N1 - Funding Information:
This research was supported by Hibah Publikasi Internasional Terindeks Tugas Akhir (Hibah PITTA B) Tahun Anggaran 2019 contract number NKB-0613/UN2.R3.1/HKP.05.00/2019 to A.O. and financially supported by Hibah Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT), Direktorat Jenderal Pendidikan Tinggi (DIKTI) 2018, Kementerian Riset Teknologi dan Pendidikan Tinggi Republik Indonesia, Research Grant, contract number 387/UN2.R3.1/HKP05.00/2018 to W.S.
Publisher Copyright:
© 2020 IOP Publishing Ltd. All rights reserved.
PY - 2020/6/12
Y1 - 2020/6/12
N2 - This study investigated aerial mycelium formation in 12 isolates of rare thermophilic Actinobacteria from Indonesia on four different media (International Streptomyces Project ISP 1, ISP 2, ISP 3, and Bennett's solidified with agar and gellan gum). The results from media solidified with agar showed that aerial mycelium formation was observed on 9 isolates as follows: 3 isolates on ISP 1 agar (Amycolatopsis and Microbispora); 3 isolates on ISP 2 agar (Amycolatopsis and Microbispora); 9 isolates on ISP 3 agar (Actinoallomurus, Amycolatopsis, Microbispora, Thermobispora, and Streptoalloteichus); and 2 isolates of Amycolatopsis on Bennett's agar. Aerial mycelium formation was not observed in 3 isolates (Microbispora and Nocardia) on all media solidified with agar. The results from media solidified with gellan gum showed that aerial mycelium formation was observed in all 12 isolates as follows: 8 isolates on ISP 1 gellan gum (Amycolatopsis, Microbispora, Nocardia and Thermobispora); 5 isolates on ISP 2 gellan gum (Amycolatopsis, Microbispora, and Nocardia); 11 isolates on ISP 3 gellan gum (Actinoallomurus, Amycolatopsis, Microbispora, Nocardia, Thermobispora, and Streptoalloteichus); and 5 isolates on Bennett's agar (Amycolatopsis, Microbispora, Nocardia, and Streptoalloeichus). These results indicate that the media solidified with gellan gum induced aerial mycelium formation in larger number of rare thermophilic Actinobacteria isolates compared to media solidified with agar.
AB - This study investigated aerial mycelium formation in 12 isolates of rare thermophilic Actinobacteria from Indonesia on four different media (International Streptomyces Project ISP 1, ISP 2, ISP 3, and Bennett's solidified with agar and gellan gum). The results from media solidified with agar showed that aerial mycelium formation was observed on 9 isolates as follows: 3 isolates on ISP 1 agar (Amycolatopsis and Microbispora); 3 isolates on ISP 2 agar (Amycolatopsis and Microbispora); 9 isolates on ISP 3 agar (Actinoallomurus, Amycolatopsis, Microbispora, Thermobispora, and Streptoalloteichus); and 2 isolates of Amycolatopsis on Bennett's agar. Aerial mycelium formation was not observed in 3 isolates (Microbispora and Nocardia) on all media solidified with agar. The results from media solidified with gellan gum showed that aerial mycelium formation was observed in all 12 isolates as follows: 8 isolates on ISP 1 gellan gum (Amycolatopsis, Microbispora, Nocardia and Thermobispora); 5 isolates on ISP 2 gellan gum (Amycolatopsis, Microbispora, and Nocardia); 11 isolates on ISP 3 gellan gum (Actinoallomurus, Amycolatopsis, Microbispora, Nocardia, Thermobispora, and Streptoalloteichus); and 5 isolates on Bennett's agar (Amycolatopsis, Microbispora, Nocardia, and Streptoalloeichus). These results indicate that the media solidified with gellan gum induced aerial mycelium formation in larger number of rare thermophilic Actinobacteria isolates compared to media solidified with agar.
KW - aerial mycelium
KW - geothermal area
KW - solidifying agent
KW - thermophilic Actinobacteria
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U2 - 10.1088/1755-1315/483/1/012017
DO - 10.1088/1755-1315/483/1/012017
M3 - Conference article
AN - SCOPUS:85086757454
SN - 1755-1307
VL - 483
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
IS - 1
M1 - 012017
T2 - 4th International Symposium on Green Technology for Value Chains 2019, GreenVC 2019
Y2 - 23 October 2019 through 24 October 2019
ER -