Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC

Shogo Taguchi; Keishi Suga; Keita Hayashi; Makoto Yoshimoto; Yukihiro Okamoto; Hidemi Nakamura; Hiroshi Umakoshi

doi:10.1016/j.colsurfb.2018.12.008

Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC

Shogo Taguchi, Keishi Suga, Keita Hayashi, Makoto Yoshimoto, Yukihiro Okamoto, Hidemi Nakamura, Hiroshi Umakoshi

ENG - Chemical Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.

Original language	English
Pages (from-to)	403-408
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	175
DOIs	https://doi.org/10.1016/j.colsurfb.2018.12.008
Publication status	Published - 2019 Mar 1

Keywords

Chlorophyll a
J-aggregate
Light-harvesting
Lipid membrane
Localization
Photo-reduction

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10.1016/j.colsurfb.2018.12.008

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@article{656a866a6f954a24a9e1ad68f3f6aeca,

title = "Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC",

abstract = "The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.",

keywords = "Chlorophyll a, J-aggregate, Light-harvesting, Lipid membrane, Localization, Photo-reduction",

author = "Shogo Taguchi and Keishi Suga and Keita Hayashi and Makoto Yoshimoto and Yukihiro Okamoto and Hidemi Nakamura and Hiroshi Umakoshi",

note = "Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) ( 26249116 ), for Scientific Research (C) ( 26420771 ), for Young Scientists (B) ( 16K18279 , 15K18279 ), and for Challenging Exploratory Research ( T15K142040 ). The author (S. T.) expresses his gratitude for the Grant-in-Aid for the Sasakawa Scientific Research Grant from the Japan Science Society , JSS ( 29-218 ). The author (K. S.) expresses his gratitude for the Multidisciplinary Research Laboratory System of Osaka University. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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doi = "10.1016/j.colsurfb.2018.12.008",

language = "English",

volume = "175",

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T1 - Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC

AU - Taguchi, Shogo

AU - Suga, Keishi

AU - Hayashi, Keita

AU - Yoshimoto, Makoto

AU - Okamoto, Yukihiro

AU - Nakamura, Hidemi

AU - Umakoshi, Hiroshi

N1 - Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aids for Scientific Research (A) ( 26249116 ), for Scientific Research (C) ( 26420771 ), for Young Scientists (B) ( 16K18279 , 15K18279 ), and for Challenging Exploratory Research ( T15K142040 ). The author (S. T.) expresses his gratitude for the Grant-in-Aid for the Sasakawa Scientific Research Grant from the Japan Science Society , JSS ( 29-218 ). The author (K. S.) expresses his gratitude for the Multidisciplinary Research Laboratory System of Osaka University. Publisher Copyright: © 2018 Elsevier B.V.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.

AB - The J-aggregate of chlorophyll a (Chla) functions as a light-harvesting antenna in natural systems. In this study, we employed the phospholipid membranes composed of longer-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and shorter-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), as a platform to induce Chla aggregates. The DMPC/DHPC assembly at the mixing ratio (q) = 1.5 induced J-aggregates of Chla at 20 °C with a total lipid concentration of 20 mM. While, Chla aggregates were not observed in the membranes at q = ∞ (DMPC vesicles) and q = 0 (DHPC micelles). The surroundings Chla molecules in DMPC/DHPC at q = 1.5 were estimated to comprise a less polar environment, based on the deconvolution analysis of Soret band spectrum (400–440 nm). The photo-reduction activity of Chla J-aggregates was investigated in lower lipid concentration conditions.

KW - Chlorophyll a

KW - J-aggregate

KW - Light-harvesting

KW - Lipid membrane

KW - Localization

KW - Photo-reduction

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U2 - 10.1016/j.colsurfb.2018.12.008

DO - 10.1016/j.colsurfb.2018.12.008

M3 - Article

C2 - 30557742

AN - SCOPUS:85058390531

SN - 0927-7765

VL - 175

SP - 403

EP - 408

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Aggregation of chlorophyll a induced in self-assembled membranes composed of DMPC and DHPC

Abstract

Keywords

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