Abstract
A procedure for producing transgenic Chinese cabbage plants by inoculating cotyledonary explants with Agrobacterium tumefaciens strain EHA101 carrying a binary vector pIG121Hm, which contains kanamycin-resistance and hygromycin-resistance genes and the GUS reporter gene, is described. Infection was most effective (highest infection frequency) when explants were infected with Agrobacterium for 15 min and co-cultivated for 3 days in co-cultivation medium at pH 5.2 supplemented with 10 mg/l acetosyringone. Transgenic plants of all three cultivars used were obtained with frequencies of 1.6-2.7% when the explants were regenerated in shoot regeneration medium solidified with 1.6% agar. A histochemical GUS assay and PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progeny showed that the transgenes were inherited in a Mendelian fashion.
| Original language | English |
|---|---|
| Pages (from-to) | 569-575 |
| Number of pages | 7 |
| Journal | Plant Cell Reports |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2000 May |
| Externally published | Yes |
Keywords
- Agrobacterium tumefaciens
- Chinese cabbage (Brassica campestris L.ssp. pekinensis)
- Cotyledonary explant
- Genetic transformation
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science