Recent results on investigations of Alfvén eigenmodes, fast ion confinement and fast ion diagnostics in JT-60U are presented. It was found that toroidicity induced Alfvén eigenmodes (TAEs) were stable in negative shear discharges with a large density gradient at the internal transport barrier (ITB). If the density gradient was small at the ITB, multiple TAEs appeared around the q = 2 surface (pitch minimum) and showed a large frequency chirping (Δf ≈ 80 kHz). In low-g positive shear discharges, the location of the TAEs changed from outside to inside the q = 1 surface, owing to a temporal change of the q profile. A significant depression of the megaelectronvolt ion population was observed only with high-n (n up to 14) multiple TAEs inside the q = 1 surface. Non-circular triangularity induced Alfvén eigenmodes were observed for the first time. Considerable depression of the triton burnup was observed in negative shear discharges. Orbit following Monte Carlo simulations indicated that ripple loss was responsible for the enhanced triton losses. The fast ion stored energies in ICRF heated negative shear discharges were comparable to those of positive shear plasmas. Tail ion temperatures in high (second to fourth) harmonic ICRF heating experiments were first analysed with an MeV neutral particle analyser. The behaviour of MeV ions produced by ICRF heating was studied with gamma ray diagnostics. A scintillating fibre detector system for detecting the 14 MeV neutron emission was developed for the triton burnup studies. Ion cyclotron emission measurements discriminating between parallel and perpendicular components of the electric field were carried out for the first time.