We newly propose a microfluidic device with multiple functions for simple microvascular connection. By using this microfluidic device with suction mechanism, we can operate the blood vessels in a single device for assisting the microvascular connection procedure such as fixing the position of the blood vessels, expanding the diameter of the blood vessels, and pouring the adhesion and drugs. We have succeeded in connecting the microcardiovascular of the extracted chicken embryonic heart to artificial mictotubes in a shorter time than the manual operation such as suturing. We have also succeeded in constructing the biological simulator that can observe a living organ continuously while circulating the culture medium through the inside of the heart. Finally, we showed the prototype platform of the evaluating the migration of the cells at the connection part between the extracted tissue from living organism and the assembled tissue structure.