Allelic loss of DNA locus of the RET proto-oncogene in small cell lung cancer

Hitoyasu Futami; Shin Ichi Egawa; Kayo Takasaki; Toshihiko Tsukada; Masahiko Shiraishi; Ken Yamaguchi

doi:10.1016/S0304-3835(03)00127-7

Allelic loss of DNA locus of the RET proto-oncogene in small cell lung cancer

Hitoyasu Futami, Shin Ichi Egawa, Kayo Takasaki, Toshihiko Tsukada, Masahiko Shiraishi, Ken Yamaguchi

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We analyzed all 21 exons of the RET proto-oncogene of paired genomic DNA from tumors and normal tissues in 12 small cell lung cancer (SCLC) patients for the presence of genetic alteration. Polymerase chain reaction single-strand conformation polymorphism analysis and direct sequencing revealed that heterozygosity of the RET proto-oncogene was lost in the tumor tissues of six patients out of eight informative SCLC patients, although point mutation was not evident in any tumors. These results suggest that a deletion of the chromosomal region including the RET proto-oncogene is involved in the pathogenesis of SCLC.

Original language	English
Pages (from-to)	59-65
Number of pages	7
Journal	Cancer Letters
Volume	195
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3835(03)00127-7
Publication status	Published - 2003 May 30
Externally published	Yes

Keywords

Loss of heterozygosity
RET
Small cell lung cancer

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0304-3835(03)00127-7

Cite this

@article{592a150f325048a3b45cfbd951520347,

title = "Allelic loss of DNA locus of the RET proto-oncogene in small cell lung cancer",

abstract = "We analyzed all 21 exons of the RET proto-oncogene of paired genomic DNA from tumors and normal tissues in 12 small cell lung cancer (SCLC) patients for the presence of genetic alteration. Polymerase chain reaction single-strand conformation polymorphism analysis and direct sequencing revealed that heterozygosity of the RET proto-oncogene was lost in the tumor tissues of six patients out of eight informative SCLC patients, although point mutation was not evident in any tumors. These results suggest that a deletion of the chromosomal region including the RET proto-oncogene is involved in the pathogenesis of SCLC.",

keywords = "Loss of heterozygosity, RET, Small cell lung cancer",

author = "Hitoyasu Futami and Egawa, {Shin Ichi} and Kayo Takasaki and Toshihiko Tsukada and Masahiko Shiraishi and Ken Yamaguchi",

note = "Funding Information: This research was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare for the Second-term Comprehensive 10-Year Strategy for Cancer Control and by Grants-in-Aid for Cancer Research (8-33 and 10-28) from the Ministry of Health and Welfare.",

year = "2003",

month = may,

day = "30",

doi = "10.1016/S0304-3835(03)00127-7",

language = "English",

volume = "195",

pages = "59--65",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Allelic loss of DNA locus of the RET proto-oncogene in small cell lung cancer

AU - Futami, Hitoyasu

AU - Egawa, Shin Ichi

AU - Takasaki, Kayo

AU - Tsukada, Toshihiko

AU - Shiraishi, Masahiko

AU - Yamaguchi, Ken

N1 - Funding Information: This research was supported in part by a Grant-in-Aid from the Ministry of Health and Welfare for the Second-term Comprehensive 10-Year Strategy for Cancer Control and by Grants-in-Aid for Cancer Research (8-33 and 10-28) from the Ministry of Health and Welfare.

PY - 2003/5/30

Y1 - 2003/5/30

N2 - We analyzed all 21 exons of the RET proto-oncogene of paired genomic DNA from tumors and normal tissues in 12 small cell lung cancer (SCLC) patients for the presence of genetic alteration. Polymerase chain reaction single-strand conformation polymorphism analysis and direct sequencing revealed that heterozygosity of the RET proto-oncogene was lost in the tumor tissues of six patients out of eight informative SCLC patients, although point mutation was not evident in any tumors. These results suggest that a deletion of the chromosomal region including the RET proto-oncogene is involved in the pathogenesis of SCLC.

AB - We analyzed all 21 exons of the RET proto-oncogene of paired genomic DNA from tumors and normal tissues in 12 small cell lung cancer (SCLC) patients for the presence of genetic alteration. Polymerase chain reaction single-strand conformation polymorphism analysis and direct sequencing revealed that heterozygosity of the RET proto-oncogene was lost in the tumor tissues of six patients out of eight informative SCLC patients, although point mutation was not evident in any tumors. These results suggest that a deletion of the chromosomal region including the RET proto-oncogene is involved in the pathogenesis of SCLC.

KW - Loss of heterozygosity

KW - RET

KW - Small cell lung cancer

UR - http://www.scopus.com/inward/record.url?scp=0038397684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038397684&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(03)00127-7

DO - 10.1016/S0304-3835(03)00127-7

M3 - Article

C2 - 12767512

AN - SCOPUS:0038397684

SN - 0304-3835

VL - 195

SP - 59

EP - 65

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Allelic loss of DNA locus of the RET proto-oncogene in small cell lung cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this