TY - JOUR
T1 - Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms
AU - Rajendar, Burki
AU - Nishizawa, Seiichi
AU - Teramae, Norio
PY - 2008
Y1 - 2008
N2 - Alloxazine can bind to adenine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-TCC AGX GCA AC-3′/3′-AGG TCN CGT TG-5′, X = AP site, N = A, T, C, G) with a dissociation constant of 0.82 μM (pH 7.0, I = 0.11 M, at 5 °C), and it is applicable to SNPs typing of PCR amplification products based on the binding-induced fluorescence response.
AB - Alloxazine can bind to adenine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-TCC AGX GCA AC-3′/3′-AGG TCN CGT TG-5′, X = AP site, N = A, T, C, G) with a dissociation constant of 0.82 μM (pH 7.0, I = 0.11 M, at 5 °C), and it is applicable to SNPs typing of PCR amplification products based on the binding-induced fluorescence response.
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U2 - 10.1039/b719786a
DO - 10.1039/b719786a
M3 - Article
C2 - 18264565
AN - SCOPUS:38949136324
SN - 1477-0520
VL - 6
SP - 670
EP - 673
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
IS - 4
ER -