Altered leukotriene b4 metabolism in colonic mucosa with inflammatory bowel disease

A. Ikehata, N. Hiwatashi, Y. Kinouchi, H. Yamazaki, K. Ito, T. Toyota

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30 Citations (Scopus)


Background: ωOxidation is regarded as the major pathway for leukotriene B4 (LTB4) metabolism. Very little is known about it in colonic mucosa with inflammatory bowel disease (IBD). Methods: We investigated the metabolic ratio of ωoxidation to LTB4 biosynthesis in colonic mucosa from patients with IBD and control subjects. After incubation of colonic mucosa with 14C-arachidonic acid and ionophore A23187, we separated LTB4 and its ωoxidative metabolites by high-performance liquid chromatography. Results: The rate of LTB4 ωoxidation was comparable to the rate of its biosynthesis. The metabolic ratio was significantly decreased in inflamed mucosa with ulcerative colitis. Conclusions: LTB4 ωhydroxylase activity is an important factor in regulating LTB4 level in colonic mucosa, and the increased LTB4 level in inflamed mucosa with IBD, especially ulcerative colitis, is caused by decreased LTB4 ωhydroxylase activity and increased 5-lipoxygenase activity.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalScandinavian Journal of Gastroenterology
Issue number1
Publication statusPublished - 1995


  • Crohn's disease
  • Ulcerative colitis


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