Amadori-glycated phosphatidylethanolamine induces lipid peroxidation in membrane model

Misa Yamada, Jeong Ho Oak, Kiyotaka Nakagawa, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review


Using large unilamellar vesicles (LUVs), generally accepted to be a biomembrane model, we investigated the peroxidative role of the Amadori-product derived from the glycation of phosphatidylethanolamine (PE), namely Amadori-PE. Amadori-PE-LUV composed of phosphatidylcholine (7 mM, PC) with Amadori-PE (0.07 mM) and PE-LUV composed of PC (7 mM) with PE (0.07 mM) were incubated in the presence of transition metal or ferritin from 0 to 6 h at 37 °C. After these reactions, structural change of LUVs was more evidently observed in Amadori-PE-LUV than in PE-LUV, and lipid hydroperoxide concentration in Amadori-PE-LUV was fourfold higher than that of the PE-LUV. In addition, Amadori-PE-induced lipid peroxidation in liposome was effectively inhibited by superoxide dismutase, mannitol, catalase and ethylenediaminetetraacetic acid (EDTA). These results indicate that Amadori-PE is a considerable risk factor, which may cause membrane lipid peroxidation in the pathogenesis of diabetes and aging.

Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalInternational Congress Series
Issue numberC
Publication statusPublished - 2002 Nov 1


  • Amadori product
  • Glycation
  • Large unilamellar vesicles
  • Lipid peroxidation
  • Phosphatidylethanolamine


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