Ammonia-assimilating microbes in microbial community in a lagoon for wastewater from paddock of dairy cattle

Hiraku Sasaki, Osamu Kitazume, Takako Sasaki, Yutaka Nakai

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    1 Citation (Scopus)


    In the present article the distribution and abundance of ammonia-assimilating microbes among the natural habitants in a lagoon were investigated. In the medium containing lagoon-extract, about 20% of total 82 isolates showed ammonia-assimilating ability. By sequencing of 16S ribosomal DNA (rDNA), the highest ammonia-assimilating isolates at 10°C and 37°C were identified as Janthinobacterium lividum and Bacillus sp., respectively. The structure of the microbial community was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Almost of the dominant species that were detected by PCR-DGGE did not coincide with isolates, which showed the high ammonia-assimilating ability, but one specie by PCR-DGGE coincided with ammonia-assimilating isolate. These results suggested that ammonia-assimilating microbes existed as non-dominant species in the microbial community in a lagoon.

    Original languageEnglish
    Pages (from-to)79-84
    Number of pages6
    JournalAnimal Science Journal
    Issue number1
    Publication statusPublished - 2004 Feb 1


    • Ammonia
    • Animal waste
    • Lagoon
    • Microbe

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)


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