TY - JOUR
T1 - Amplification of c-Ki-ras gene and aberrant expression of c-myc in WI-38 cells transformed in vitro by γ-irradiation
AU - Mizuki, Kiyoshi
AU - Nose, Kiyoshi
AU - Okamoto, Hiroshi
AU - Tsuchida, Nobuo
AU - Hayashi, Kenshi
N1 - Funding Information:
Acknowledgements This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture. The authors are grateful to Dr. G. Soma of Meiji Pharmaceutical College and to Drs. T. Yamamoto and K. Toyoshima of Institute of Medical Science, University of Tokyo for providing the E, yes and myc probes. The HpCl and pHFSA-1 probes were kindly supplied by Dr. Dalgleish, N.I.H. and by Dr. Gunning, Stanford Univ. School of Medicine, respectively.
PY - 1985/4/30
Y1 - 1985/4/30
N2 - The expression of c-oncogenes was examined with normal human fibroblasts (WI-38) and the cells transformed in vitro by γ-irradiation (CT-1). The amount of Ki-ras-specific mRNA in CT-1 cells was found to be approximately twice that in WI-38 cells. By Southern blot hybridization, the c-Ki-ras 2 gene of CT-1 cells was found to be amplified about two-fold. CT-1 cells have higher levels of c-myc mRNA than normal cells, although the gene dosage and the restriction nuclease pattern of the c-myc gene was the same. The content of c-myc mRNA in CT-1 cells was found to be constitutively high, whereas that in normal cells was increased by serum addition.
AB - The expression of c-oncogenes was examined with normal human fibroblasts (WI-38) and the cells transformed in vitro by γ-irradiation (CT-1). The amount of Ki-ras-specific mRNA in CT-1 cells was found to be approximately twice that in WI-38 cells. By Southern blot hybridization, the c-Ki-ras 2 gene of CT-1 cells was found to be amplified about two-fold. CT-1 cells have higher levels of c-myc mRNA than normal cells, although the gene dosage and the restriction nuclease pattern of the c-myc gene was the same. The content of c-myc mRNA in CT-1 cells was found to be constitutively high, whereas that in normal cells was increased by serum addition.
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U2 - 10.1016/0006-291X(85)90152-4
DO - 10.1016/0006-291X(85)90152-4
M3 - Article
C2 - 2581563
AN - SCOPUS:0021856104
SN - 0006-291X
VL - 128
SP - 1037
EP - 1043
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -