An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

Kazumitsu Onizuka; Takamasa Nishioka; Zhichun Li; Daichi Jitsuzaki; Yosuke Taniguchi; Shigeki Sasaki

doi:10.1039/c2cc17621a

An efficient and simple method for site-selective modification of O⁶-methyl-2′-deoxyguanosine in DNA

Kazumitsu Onizuka, Takamasa Nishioka, Zhichun Li, Daichi Jitsuzaki, Yosuke Taniguchi, Shigeki Sasaki

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

O⁶-Methyl-2′-deoxyguanosine (O⁶-Me-dG) is a mutagenic nucleotide in DNA. O⁶-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O⁶-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O⁶-Me-dG in DNA.

Original language	English
Pages (from-to)	3969-3971
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	33
DOIs	https://doi.org/10.1039/c2cc17621a
Publication status	Published - 2012 Mar 26
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c2cc17621a

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abstract = "O6-Methyl-2′-deoxyguanosine (O6-Me-dG) is a mutagenic nucleotide in DNA. O6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O6-Me-dG in DNA.",

author = "Kazumitsu Onizuka and Takamasa Nishioka and Zhichun Li and Daichi Jitsuzaki and Yosuke Taniguchi and Shigeki Sasaki",

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T1 - An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

AU - Onizuka, Kazumitsu

AU - Nishioka, Takamasa

AU - Li, Zhichun

AU - Jitsuzaki, Daichi

AU - Taniguchi, Yosuke

AU - Sasaki, Shigeki

PY - 2012/3/26

Y1 - 2012/3/26

N2 - O6-Methyl-2′-deoxyguanosine (O6-Me-dG) is a mutagenic nucleotide in DNA. O6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O6-Me-dG in DNA.

AB - O6-Methyl-2′-deoxyguanosine (O6-Me-dG) is a mutagenic nucleotide in DNA. O6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O6-Me-dG in DNA.

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JO - Chemical Communications

JF - Chemical Communications

IS - 33

ER -

An efficient and simple method for site-selective modification of O⁶-methyl-2′-deoxyguanosine in DNA

Abstract

ASJC Scopus subject areas

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