TY - GEN
T1 - An electrochemical biosensor based on gold microspheres and nanoporous gold for real-time detection of superoxide anion in skeletal muscle tissue
AU - Sadeghian, Ramin Banan
AU - Ostrovidov, Serge
AU - Salehi, Sahar
AU - Han, Jiuhui
AU - Chen, Mingwei
AU - Khademhosseini, Ali
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/11/4
Y1 - 2015/11/4
N2 - Superoxide anion (SOA) as a member of reactive oxygen species (ROS) group is involved in various physiological and pathological states. For instance, generation of SOA is known to increase with skeletal muscle contractile activity and fatigue. It is therefore important to selectively detect and accurately quantify the release of SOA within both physiological and pathological levels. We report fabrication and characterization of a cytochrome-c functionalized SOA biosensor built on commercially available miniaturized screen-printed electrodes made of gold microspheres. The device was first tested and calibrated in a xanthine/xanthine oxidase (XOD) system and then employed to detect SOA release from C2C12 myoblasts and myotubes upon stimulation with PMA.
AB - Superoxide anion (SOA) as a member of reactive oxygen species (ROS) group is involved in various physiological and pathological states. For instance, generation of SOA is known to increase with skeletal muscle contractile activity and fatigue. It is therefore important to selectively detect and accurately quantify the release of SOA within both physiological and pathological levels. We report fabrication and characterization of a cytochrome-c functionalized SOA biosensor built on commercially available miniaturized screen-printed electrodes made of gold microspheres. The device was first tested and calibrated in a xanthine/xanthine oxidase (XOD) system and then employed to detect SOA release from C2C12 myoblasts and myotubes upon stimulation with PMA.
UR - http://www.scopus.com/inward/record.url?scp=84953247584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84953247584&partnerID=8YFLogxK
U2 - 10.1109/EMBC.2015.7320239
DO - 10.1109/EMBC.2015.7320239
M3 - Conference contribution
C2 - 26738139
AN - SCOPUS:84953247584
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 7962
EP - 7965
BT - 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
Y2 - 25 August 2015 through 29 August 2015
ER -