An improved mounting method for observation of thick specimens using confocal microscopy

M. Kagayama; Y. Sasano; M. Hirata; I. Mizoguchi; I. Takahashi

doi:10.3109/10520299609117165

An improved mounting method for observation of thick specimens using confocal microscopy

M. Kagayama, Y. Sasano, M. Hirata, I. Mizoguchi, I. Takahashi

DEN - Dental Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.

Original language	English
Pages (from-to)	231-233
Number of pages	3
Journal	Biotechnic and Histochemistry
Volume	71
Issue number	5
DOIs	https://doi.org/10.3109/10520299609117165
Publication status	Published - 1996 Sept

Keywords

Spurr's resin
confocal microscopy
fluorescence
mounting method
whole mount specimen

ASJC Scopus subject areas

Histology
Medical Laboratory Technology

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10.3109/10520299609117165

Cite this

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title = "An improved mounting method for observation of thick specimens using confocal microscopy",

abstract = "When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.",

keywords = "Spurr's resin, confocal microscopy, fluorescence, mounting method, whole mount specimen",

author = "M. Kagayama and Y. Sasano and M. Hirata and I. Mizoguchi and I. Takahashi",

note = "Funding Information: We thank Mr. M. Eguchi and Mr. Y. Mikami for their technical assistance. This study was supported in part by grants-in-aid (05557077, 0757282, and 07557283) from the Ministry of Science, Education and Culture of Japan.",

year = "1996",

month = sep,

doi = "10.3109/10520299609117165",

language = "English",

volume = "71",

pages = "231--233",

journal = "Biotechnic and Histochemistry",

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TY - JOUR

T1 - An improved mounting method for observation of thick specimens using confocal microscopy

AU - Kagayama, M.

AU - Sasano, Y.

AU - Hirata, M.

AU - Mizoguchi, I.

AU - Takahashi, I.

N1 - Funding Information: We thank Mr. M. Eguchi and Mr. Y. Mikami for their technical assistance. This study was supported in part by grants-in-aid (05557077, 0757282, and 07557283) from the Ministry of Science, Education and Culture of Japan.

PY - 1996/9

Y1 - 1996/9

N2 - When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.

AB - When applying aqueous media to mount thick specimens, air bubbles occur frequently between coverslips and slide glasses. This results in an increase of background signal by confocal microscopy. Using Spurr's resin as a mounting medium, we could observe thick specimens with oil immersion objective lens without the use of coverslips, then avoid air bubbles near the specimen. Fading of the fluorescence appeared to be less in specimens mounted by the resin than in specimens mounted by aqueous media. Further, the new method increased depth of field so that more planes of specimens could be analyzed.

KW - Spurr's resin

KW - confocal microscopy

KW - fluorescence

KW - mounting method

KW - whole mount specimen

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JO - Biotechnic and Histochemistry

JF - Biotechnic and Histochemistry

IS - 5

ER -

An improved mounting method for observation of thick specimens using confocal microscopy

Abstract

Keywords

ASJC Scopus subject areas

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