An interaction between p21ras and heat shock protein hsp60, a chaperonin

Shuntaro Ikawa, Robert A. Weinberg

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65 Citations (Scopus)


Ras proteins play a crucial role in the development of neoplasia and in signal transduction in normal cells. In a search for proteins interacting with p21ras, we previously identified a protein of 60 kDa (p60) through use of a chemical cross-linker. Using information from partial amino acid sequencing of the purified protein, we isolated full-length cDNA clones encoding this 60-kDa protein. Nucleotide sequence analysis revealed that p60 is the murine heat shock protein hsp60, a chaperonin. Association of hsp60 with p21ras appears physiological, as the amount of hsp60 complexed to p21ras was similar even in cells over-expressing p21ras, and reversing the order of cross-linking and lysis of the cells, which releases large amounts of hsp60 from mitochondria, did not alter the amount of hsp60 cross-linked to p21ras.

Original languageEnglish
Pages (from-to)2012-2016
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
Publication statusPublished - 1992


  • Molecular chaperon
  • Oncogenes
  • Signal transduction


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