An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies

Hidetoshi Sato; Toshiaki Suzuki; Bibin B. Andriana; Shin'ichi Morita; Atsushi Maruyama; Hideyuki Shinzawa; Yuichi Komachi; Gen'ichi Kanai; Nobuo Ura; Koji Masutani; Yuji Matsuura; Masakazu Toi; Toru Shimosegawa; Yukihiro Ozaki

doi:10.1117/12.809271

An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies

Hidetoshi Sato, Toshiaki Suzuki, Bibin B. Andriana, Shin'ichi Morita, Atsushi Maruyama, Hideyuki Shinzawa, Yuichi Komachi, Gen'ichi Kanai, Nobuo Ura, Koji Masutani, Yuji Matsuura, Masakazu Toi, Toru Shimosegawa, Yukihiro Ozaki

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

An optical biopsy system which equips miniaturized Raman probes, a miniaturized endoscope and a fluorescent image probe has been developed for in vivo studies of live experimental animals. The present report describes basic optical properties of the system and its application studies for in vivo cancer model animals and ex vivo human cancer tissues. It was developed two types of miniaturized Raman probes, micro Raman probe (MRP) made of optical fibers and ball lens hollow optical fiber Raman probe (BHRP) made of single hollow optical fiber (HOF) with a ball lens. The former has rather large working distance (WD), up to one millimeter. The latter has small WD (∼300μm) which depends on the focal length of the ball lens. Use of multiple probes with different WD allows one to obtain detailed information of subsurface tissues in the totally noninvasive manner. The probe is enough narrow to be inserted into a biopsy needle (∼19G), for observations of the lesion at deeper inside bodies. The miniaturized endoscope has been applied to observe progression of a stomach cancer in the same rat lesion. It was succeeded to visualize structure of non-stained cancer tissue in live model animals by the fluorescent image technique. The system was also applied to ex vivo studies of human breast and stomach cancers.

Original language	English
Article number	71690M
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7169
DOIs	https://doi.org/10.1117/12.809271
Publication status	Published - 2009
Event	Advanced Biomedical and Clinical Diagnostic Systems VII - San Jose, CA, United States Duration: 2009 Jan 25 → 2009 Jan 26

Keywords

Fluorescence
Hollow optical fiber
In vivo measurement
Miniaturized Raman probe
Raman spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.809271

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Sato, H., Suzuki, T., Andriana, B. B., Morita, S., Maruyama, A., Shinzawa, H., Komachi, Y., Kanai, G., Ura, N., Masutani, K., Matsuura, Y., Toi, M., Shimosegawa, T., & Ozaki, Y. (2009). An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7169, Article 71690M. https://doi.org/10.1117/12.809271

@article{2523f448a9c347a5b5c00029b9c0a1f0,

title = "An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies",

abstract = "An optical biopsy system which equips miniaturized Raman probes, a miniaturized endoscope and a fluorescent image probe has been developed for in vivo studies of live experimental animals. The present report describes basic optical properties of the system and its application studies for in vivo cancer model animals and ex vivo human cancer tissues. It was developed two types of miniaturized Raman probes, micro Raman probe (MRP) made of optical fibers and ball lens hollow optical fiber Raman probe (BHRP) made of single hollow optical fiber (HOF) with a ball lens. The former has rather large working distance (WD), up to one millimeter. The latter has small WD (∼300μm) which depends on the focal length of the ball lens. Use of multiple probes with different WD allows one to obtain detailed information of subsurface tissues in the totally noninvasive manner. The probe is enough narrow to be inserted into a biopsy needle (∼19G), for observations of the lesion at deeper inside bodies. The miniaturized endoscope has been applied to observe progression of a stomach cancer in the same rat lesion. It was succeeded to visualize structure of non-stained cancer tissue in live model animals by the fluorescent image technique. The system was also applied to ex vivo studies of human breast and stomach cancers.",

keywords = "Fluorescence, Hollow optical fiber, In vivo measurement, Miniaturized Raman probe, Raman spectroscopy",

author = "Hidetoshi Sato and Toshiaki Suzuki and Andriana, {Bibin B.} and Shin'ichi Morita and Atsushi Maruyama and Hideyuki Shinzawa and Yuichi Komachi and Gen'ichi Kanai and Nobuo Ura and Koji Masutani and Yuji Matsuura and Masakazu Toi and Toru Shimosegawa and Yukihiro Ozaki",

year = "2009",

doi = "10.1117/12.809271",

language = "English",

volume = "7169",

journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

issn = "1605-7422",

publisher = "SPIE",

note = "Advanced Biomedical and Clinical Diagnostic Systems VII ; Conference date: 25-01-2009 Through 26-01-2009",

}

Sato, H, Suzuki, T, Andriana, BB, Morita, S, Maruyama, A, Shinzawa, H, Komachi, Y, Kanai, G, Ura, N, Masutani, K, Matsuura, Y, Toi, M, Shimosegawa, T & Ozaki, Y 2009, 'An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies', Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7169, 71690M. https://doi.org/10.1117/12.809271

An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies. / Sato, Hidetoshi; Suzuki, Toshiaki; Andriana, Bibin B. et al.
In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 7169, 71690M, 2009.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies

AU - Sato, Hidetoshi

AU - Suzuki, Toshiaki

AU - Andriana, Bibin B.

AU - Morita, Shin'ichi

AU - Maruyama, Atsushi

AU - Shinzawa, Hideyuki

AU - Komachi, Yuichi

AU - Kanai, Gen'ichi

AU - Ura, Nobuo

AU - Masutani, Koji

AU - Matsuura, Yuji

AU - Toi, Masakazu

AU - Shimosegawa, Toru

AU - Ozaki, Yukihiro

PY - 2009

Y1 - 2009

N2 - An optical biopsy system which equips miniaturized Raman probes, a miniaturized endoscope and a fluorescent image probe has been developed for in vivo studies of live experimental animals. The present report describes basic optical properties of the system and its application studies for in vivo cancer model animals and ex vivo human cancer tissues. It was developed two types of miniaturized Raman probes, micro Raman probe (MRP) made of optical fibers and ball lens hollow optical fiber Raman probe (BHRP) made of single hollow optical fiber (HOF) with a ball lens. The former has rather large working distance (WD), up to one millimeter. The latter has small WD (∼300μm) which depends on the focal length of the ball lens. Use of multiple probes with different WD allows one to obtain detailed information of subsurface tissues in the totally noninvasive manner. The probe is enough narrow to be inserted into a biopsy needle (∼19G), for observations of the lesion at deeper inside bodies. The miniaturized endoscope has been applied to observe progression of a stomach cancer in the same rat lesion. It was succeeded to visualize structure of non-stained cancer tissue in live model animals by the fluorescent image technique. The system was also applied to ex vivo studies of human breast and stomach cancers.

AB - An optical biopsy system which equips miniaturized Raman probes, a miniaturized endoscope and a fluorescent image probe has been developed for in vivo studies of live experimental animals. The present report describes basic optical properties of the system and its application studies for in vivo cancer model animals and ex vivo human cancer tissues. It was developed two types of miniaturized Raman probes, micro Raman probe (MRP) made of optical fibers and ball lens hollow optical fiber Raman probe (BHRP) made of single hollow optical fiber (HOF) with a ball lens. The former has rather large working distance (WD), up to one millimeter. The latter has small WD (∼300μm) which depends on the focal length of the ball lens. Use of multiple probes with different WD allows one to obtain detailed information of subsurface tissues in the totally noninvasive manner. The probe is enough narrow to be inserted into a biopsy needle (∼19G), for observations of the lesion at deeper inside bodies. The miniaturized endoscope has been applied to observe progression of a stomach cancer in the same rat lesion. It was succeeded to visualize structure of non-stained cancer tissue in live model animals by the fluorescent image technique. The system was also applied to ex vivo studies of human breast and stomach cancers.

KW - Fluorescence

KW - Hollow optical fiber

KW - In vivo measurement

KW - Miniaturized Raman probe

KW - Raman spectroscopy

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U2 - 10.1117/12.809271

DO - 10.1117/12.809271

M3 - Conference article

AN - SCOPUS:66249147564

SN - 1605-7422

VL - 7169

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 71690M

T2 - Advanced Biomedical and Clinical Diagnostic Systems VII

Y2 - 25 January 2009 through 26 January 2009

ER -

An optical biopsy system with miniaturized raman and spectral imaging probes; in vivo animal and ex vivo clinical application studies

Abstract

Keywords

UN SDGs

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