An oxygen responsive microparticle-patterned hydrogel sheet for enzyme activity imaging

Kuniaki Nagamine; Shuntaro Ito; Mai Takeda; Shingo Otani; Matsuhiko Nishizawa

doi:10.5796/electrochemistry.80.318

An oxygen responsive microparticle-patterned hydrogel sheet for enzyme activity imaging

Kuniaki Nagamine, Shuntaro Ito, Mai Takeda, Shingo Otani, Matsuhiko Nishizawa

ENG - Finemechanics

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible Polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.

Original language	English
Pages (from-to)	318-320
Number of pages	3
Journal	Electrochemistry
Volume	80
Issue number	5
DOIs	https://doi.org/10.5796/electrochemistry.80.318
Publication status	Published - 2012 May

Keywords

Electrohydrodynamics
Microfluidic device
Oxygen imaging sheet

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10.5796/electrochemistry.80.318

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abstract = "A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible Polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.",

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author = "Kuniaki Nagamine and Shuntaro Ito and Mai Takeda and Shingo Otani and Matsuhiko Nishizawa",

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AU - Nagamine, Kuniaki

AU - Ito, Shuntaro

AU - Takeda, Mai

AU - Otani, Shingo

AU - Nishizawa, Matsuhiko

PY - 2012/5

Y1 - 2012/5

N2 - A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible Polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.

AB - A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible Polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.

KW - Electrohydrodynamics

KW - Microfluidic device

KW - Oxygen imaging sheet

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M3 - Article

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SP - 318

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JO - Electrochemistry

JF - Electrochemistry

IS - 5

ER -

An oxygen responsive microparticle-patterned hydrogel sheet for enzyme activity imaging

Abstract

Keywords

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