TY - JOUR
T1 - An ultramicroelectrode for determination of intracellular oxygen. Light-irradiation-induced change in oxygen concentration in an algal protoplast
AU - Matsue, Tomokazu
AU - Koike, Shinji
AU - Abe, Takayuki
AU - Itabashi, Takeyuki
AU - Uchida, Isamu
PY - 1992/7/6
Y1 - 1992/7/6
N2 - An oxygen ultramicroelectrode was fabricated by pyrolysis of butane inside a glass capillary, followed by electrodeposition of Pt. The intracellular determination of oxygen in an algal protoplast (diameter, approx. 80 μm) with the ultramicroelectrode indicated that at a light intensity of 25 klx, the oxygen efflux from a single protoplast caused by the light reactions is approx. 5·10-13 mol/s and that corresponding to the dark reactions approx, 1 · 10-12 mol/s.
AB - An oxygen ultramicroelectrode was fabricated by pyrolysis of butane inside a glass capillary, followed by electrodeposition of Pt. The intracellular determination of oxygen in an algal protoplast (diameter, approx. 80 μm) with the ultramicroelectrode indicated that at a light intensity of 25 klx, the oxygen efflux from a single protoplast caused by the light reactions is approx. 5·10-13 mol/s and that corresponding to the dark reactions approx, 1 · 10-12 mol/s.
KW - Alga
KW - Intracellular oxygen
KW - Light irradiation
KW - Oxygen
KW - Protoplast
KW - Ultramicroelectrode
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U2 - 10.1016/0167-4838(92)90468-S
DO - 10.1016/0167-4838(92)90468-S
M3 - Article
AN - SCOPUS:0026632621
SN - 0167-4838
VL - 1101
SP - 69
EP - 72
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 1
ER -