Anaerobic nitrogen-fixing consortia consisting of clostridia isolated from gramineous plants

Kiwamu Minamisawa, Kiyo Nishioka, Taro Miyaki, Bin Ye, Takuya Miyamoto, Mu You, Asami Saito, Masanori Saito, Wilfredo L. Barraquio, Neung Teaumroong, Than Sein, Tadashi Sato

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)


We report here the existence of anaerobic nitrogen-fixing consortia (ANFICOs) consisting of N2-fixing clostridia and diverse nondiazotrophic bacteria in nonleguminous plants; we found these ANFICOs while attempting to overcome a problem with culturing nitrogen-fixing microbes from various gramineous plants. A major feature of ANFICOs is that N2 fixation by the anaerobic clostridia is supported by the elimination of oxygen by the accompanying bacteria in the culture. In a few ANFICOs, nondiazotrophic bacteria specifically induced nitrogen fixation of the clostridia in culture. ANFICOs are widespread in wild rice species and pioneer plants, which are able to grow in unfavorable locations. These results indicate that clostridia are naturally occurring endophytes in gramineous plants and that clostridial N 2 fixation arises in association with nondiazotrophic endophytes.

Original languageEnglish
Pages (from-to)3096-3102
Number of pages7
JournalApplied and Environmental Microbiology
Issue number5
Publication statusPublished - 2004 May


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