Analysis for localization of steroid sulfatase in human tissues

Takashi Suzuki; Yasuhiro Miki; Tsuyoshi Fukuda; Taisuke Nakata; Takuya Moriya; Hironobu Sasano

doi:10.1016/S0076-6879(05)00018-2

Analysis for localization of steroid sulfatase in human tissues

Takashi Suzuki, Yasuhiro Miki, Tsuyoshi Fukuda, Taisuke Nakata, Takuya Moriya, Hironobu Sasano

MED - Medical Sciences

Research output: Contribution to journal › Review article › peer-review

9 Citations (Scopus)

Abstract

Human steroid sulfatase (STS) is an enzyme that hydrolyzes several sulfated steroids, such as estrone sulfate, dehydroepiandrosterone sulfate, and cholesterol sulfate, and results in the production of active substances. STS has been demonstrated in human breast cancer tissues and is considered to be involved in intratumoral estrogen production. It is very important to analyze the cellular distribution of STS with accuracy in human tissues in order to obtain a better understanding of the biological significance of STS. Therefore, this chapter describes several morphological approaches used to study the localization of STS, including immunohistochemistry, mRNA in situ hybridization, and laser capture microdissection/reverse transcription-polymerase chain reaction, in human tissues.

Original language	English
Article number	18
Pages (from-to)	303-316
Number of pages	14
Journal	Methods in Enzymology
Volume	400
DOIs	https://doi.org/10.1016/S0076-6879(05)00018-2
Publication status	Published - 2005

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10.1016/S0076-6879(05)00018-2

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title = "Analysis for localization of steroid sulfatase in human tissues",

abstract = "Human steroid sulfatase (STS) is an enzyme that hydrolyzes several sulfated steroids, such as estrone sulfate, dehydroepiandrosterone sulfate, and cholesterol sulfate, and results in the production of active substances. STS has been demonstrated in human breast cancer tissues and is considered to be involved in intratumoral estrogen production. It is very important to analyze the cellular distribution of STS with accuracy in human tissues in order to obtain a better understanding of the biological significance of STS. Therefore, this chapter describes several morphological approaches used to study the localization of STS, including immunohistochemistry, mRNA in situ hybridization, and laser capture microdissection/reverse transcription-polymerase chain reaction, in human tissues.",

author = "Takashi Suzuki and Yasuhiro Miki and Tsuyoshi Fukuda and Taisuke Nakata and Takuya Moriya and Hironobu Sasano",

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T1 - Analysis for localization of steroid sulfatase in human tissues

AU - Suzuki, Takashi

AU - Miki, Yasuhiro

AU - Fukuda, Tsuyoshi

AU - Nakata, Taisuke

AU - Moriya, Takuya

AU - Sasano, Hironobu

PY - 2005

Y1 - 2005

N2 - Human steroid sulfatase (STS) is an enzyme that hydrolyzes several sulfated steroids, such as estrone sulfate, dehydroepiandrosterone sulfate, and cholesterol sulfate, and results in the production of active substances. STS has been demonstrated in human breast cancer tissues and is considered to be involved in intratumoral estrogen production. It is very important to analyze the cellular distribution of STS with accuracy in human tissues in order to obtain a better understanding of the biological significance of STS. Therefore, this chapter describes several morphological approaches used to study the localization of STS, including immunohistochemistry, mRNA in situ hybridization, and laser capture microdissection/reverse transcription-polymerase chain reaction, in human tissues.

AB - Human steroid sulfatase (STS) is an enzyme that hydrolyzes several sulfated steroids, such as estrone sulfate, dehydroepiandrosterone sulfate, and cholesterol sulfate, and results in the production of active substances. STS has been demonstrated in human breast cancer tissues and is considered to be involved in intratumoral estrogen production. It is very important to analyze the cellular distribution of STS with accuracy in human tissues in order to obtain a better understanding of the biological significance of STS. Therefore, this chapter describes several morphological approaches used to study the localization of STS, including immunohistochemistry, mRNA in situ hybridization, and laser capture microdissection/reverse transcription-polymerase chain reaction, in human tissues.

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JO - Methods in Enzymology

JF - Methods in Enzymology

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Analysis for localization of steroid sulfatase in human tissues

Abstract

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