Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry

Seon Hwa Lee; Tomoyuki Oe; Ian A. Blair

Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry

Seon Hwa Lee, Tomoyuki Oe, Ian A. Blair

PHA - Life and Pharmaceutical Science

University of Pennsylvania

Research output: Contribution to conference › Paper › peer-review

Abstract

The development of an assay for the analysis of etheno 2′- Deoxygyanosine (dGuo) adducts from biological matrices was described. The use of isotope dilution liquid chromatography/atomospheric pressure chemical ionization/mass spectrometry (LC/APCI/MA) was also discussed. The reaction between 13-hydroperoxyoctadecadienoic acid (13-HPODE) and dGuo in the presence of vitamin C resulted in the fomation of three products; etheno-dGuo, carboxynonanone etheno-dGuo, and heptanone ehteno-dGuo. The results show that the mass spectrum of the most polar adduct (10.4 min) revealed an intense protonated molecular ion (H ⁺) along with a BH ₂ ⁺ ion at m/z 176.

Original language	English
Pages	635-636
Number of pages	2
Publication status	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2002 Jun 2 → 2002 Jun 6

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	02/6/2 → 02/6/6

Cite this

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title = "Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry",

abstract = "The development of an assay for the analysis of etheno 2′- Deoxygyanosine (dGuo) adducts from biological matrices was described. The use of isotope dilution liquid chromatography/atomospheric pressure chemical ionization/mass spectrometry (LC/APCI/MA) was also discussed. The reaction between 13-hydroperoxyoctadecadienoic acid (13-HPODE) and dGuo in the presence of vitamin C resulted in the fomation of three products; etheno-dGuo, carboxynonanone etheno-dGuo, and heptanone ehteno-dGuo. The results show that the mass spectrum of the most polar adduct (10.4 min) revealed an intense protonated molecular ion (H +) along with a BH 2 + ion at m/z 176.",

author = "Lee, {Seon Hwa} and Tomoyuki Oe and Blair, {Ian A.}",

year = "2002",

language = "English",

pages = "635--636",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry. / Lee, Seon Hwa ; Oe, Tomoyuki; Blair, Ian A.
2002. 635-636 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry

AU - Lee, Seon Hwa

AU - Oe, Tomoyuki

AU - Blair, Ian A.

PY - 2002

Y1 - 2002

N2 - The development of an assay for the analysis of etheno 2′- Deoxygyanosine (dGuo) adducts from biological matrices was described. The use of isotope dilution liquid chromatography/atomospheric pressure chemical ionization/mass spectrometry (LC/APCI/MA) was also discussed. The reaction between 13-hydroperoxyoctadecadienoic acid (13-HPODE) and dGuo in the presence of vitamin C resulted in the fomation of three products; etheno-dGuo, carboxynonanone etheno-dGuo, and heptanone ehteno-dGuo. The results show that the mass spectrum of the most polar adduct (10.4 min) revealed an intense protonated molecular ion (H +) along with a BH 2 + ion at m/z 176.

AB - The development of an assay for the analysis of etheno 2′- Deoxygyanosine (dGuo) adducts from biological matrices was described. The use of isotope dilution liquid chromatography/atomospheric pressure chemical ionization/mass spectrometry (LC/APCI/MA) was also discussed. The reaction between 13-hydroperoxyoctadecadienoic acid (13-HPODE) and dGuo in the presence of vitamin C resulted in the fomation of three products; etheno-dGuo, carboxynonanone etheno-dGuo, and heptanone ehteno-dGuo. The results show that the mass spectrum of the most polar adduct (10.4 min) revealed an intense protonated molecular ion (H +) along with a BH 2 + ion at m/z 176.

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M3 - Paper

AN - SCOPUS:2442694530

SP - 635

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T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Analysis of etheno 2′-deoxyguanosine adducts as a dosimeter for oxidative stress by liquid chromatography/atmospheric pressure chemical ionization/mass spectrometry

Abstract

Conference

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