TY - JOUR
T1 - Analysis of expressed sequence tags of flower buds in Lotus japonicus
AU - Endo, Makoto
AU - Kokubun, Takao
AU - Takahata, Yoshihito
AU - Higashitani, Atsushi
AU - Tabata, Satoshi
AU - Watanabe, Masao
PY - 2000
Y1 - 2000
N2 - In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomly selected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L. japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.
AB - In order to study gene expression in a reproductive organ, we constructed a cDNA library of mature flower buds in Lotus japonicus, and characterized expressed sequence tags (ESTs) of 842 clones randomly selected. The EST sequences were clustered into 718 non-redundant groups. From BLAST and FASTA search analyses of both protein and DNA databases, 58.5% of the EST groups showed significant sequence similarities to known genes. Several genes encoding these EST clones were identified as pollen-specific genes, such as pectin methylesterase, ascorbate oxidase, and polygalacturonase, and as homologous genes involved in pollen-pistil interaction. Comparison of these EST sequences with those derived from the whole plant of L. japonicus, revealed that 64.8% of EST sequences from the flower buds were not found in EST sequences of the whole plant. Taken together, the EST data from flower buds generated in this study is useful in dissecting gene expression in floral organ of L. japonicus.
KW - ESTs
KW - Flower bud
KW - Legume
KW - Lotus japonicus
KW - Pollen-specific gene
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U2 - 10.1093/dnares/7.3.213
DO - 10.1093/dnares/7.3.213
M3 - Article
C2 - 10907852
AN - SCOPUS:0034733174
SN - 1340-2838
VL - 7
SP - 213
EP - 216
JO - DNA Research
JF - DNA Research
IS - 3
ER -