Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray

Itaru Nishizuka; Takashi Ishikawa; Yohei Hamaguchi; Masako Kamiyama; Yasushi Ichikawa; Koji Kadota; Rika Miki; Yasuhiro Tomaru; Yosuke Mizuno; Naoko Tominaga; Rieko Yano; Hitoshi Goto; Hiroyuki Nitanda; Shinji Togo; Yasushi Okazaki; Yoshihide Hayashizaki; Hiroshi Shimada

doi:10.1007/BF02967543

Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray

Itaru Nishizuka, Takashi Ishikawa, Yohei Hamaguchi, Masako Kamiyama, Yasushi Ichikawa, Koji Kadota, Rika Miki, Yasuhiro Tomaru, Yosuke Mizuno, Naoko Tominaga, Rieko Yano, Hitoshi Goto, Hiroyuki Nitanda, Shinji Togo, Yasushi Okazaki, Yoshihide Hayashizaki, Hiroshi Shimada

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Background: Brain metastases occur in 15% to 30% of breast cancer patients, usually as a late event. The patterns of metastases to different organs are determined by the tumor cell phenotype and interactions between the tumor cells and the organ environment. Methods: We investigated the gene expression profile occurring in brain metastases from a breast cancer cell line. We used cDNA microarrays to compare patterns of gene expression between the mouse breast cancer cell line Jyg MC (A) and a subline that often metastasis to brain, (B). Results: By Microarray analysis about 350 of 21,000 genes were significantly up-regulated in Jyg MC (B). Many candidate genes that may be associated with the establishment of brain metastasis from breast cancer were included. Interestingly, we found that the expression of astrocyte derived cytokine receptors (IL-6 receptor, TGF-beta receptor and IGF receptor) were significantly increased in Jyg MC (B) cells. These results were confirmed by RT-PCR. Conclusion: These results suggest that cytokines produced by glial cells in vivo may contribute, in a paracrine manner, to the development of brain metastases from breast cancer cells.

Original language	English
Pages (from-to)	26-32
Number of pages	7
Journal	Breast Cancer
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/BF02967543
Publication status	Published - 2002 Jan
Externally published	Yes

Keywords

Brain metastasis
Breast cancer
CDNA microarray
Cell line
Cytokine

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02967543

Cite this

Nishizuka, I., Ishikawa, T., Hamaguchi, Y., Kamiyama, M., Ichikawa, Y., Kadota, K., Miki, R., Tomaru, Y., Mizuno, Y., Tominaga, N., Yano, R., Goto, H., Nitanda, H., Togo, S., Okazaki, Y., Hayashizaki, Y., & Shimada, H. (2002). Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray. Breast Cancer, 9(1), 26-32. https://doi.org/10.1007/BF02967543

Nishizuka, I, Ishikawa, T, Hamaguchi, Y, Kamiyama, M, Ichikawa, Y, Kadota, K, Miki, R, Tomaru, Y, Mizuno, Y, Tominaga, N, Yano, R, Goto, H, Nitanda, H, Togo, S, Okazaki, Y, Hayashizaki, Y & Shimada, H 2002, 'Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray', Breast Cancer, vol. 9, no. 1, pp. 26-32. https://doi.org/10.1007/BF02967543

@article{3458c49086db42ba803d3156480833c4,

title = "Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray",

abstract = "Background: Brain metastases occur in 15% to 30% of breast cancer patients, usually as a late event. The patterns of metastases to different organs are determined by the tumor cell phenotype and interactions between the tumor cells and the organ environment. Methods: We investigated the gene expression profile occurring in brain metastases from a breast cancer cell line. We used cDNA microarrays to compare patterns of gene expression between the mouse breast cancer cell line Jyg MC (A) and a subline that often metastasis to brain, (B). Results: By Microarray analysis about 350 of 21,000 genes were significantly up-regulated in Jyg MC (B). Many candidate genes that may be associated with the establishment of brain metastasis from breast cancer were included. Interestingly, we found that the expression of astrocyte derived cytokine receptors (IL-6 receptor, TGF-beta receptor and IGF receptor) were significantly increased in Jyg MC (B) cells. These results were confirmed by RT-PCR. Conclusion: These results suggest that cytokines produced by glial cells in vivo may contribute, in a paracrine manner, to the development of brain metastases from breast cancer cells.",

keywords = "Brain metastasis, Breast cancer, CDNA microarray, Cell line, Cytokine",

author = "Itaru Nishizuka and Takashi Ishikawa and Yohei Hamaguchi and Masako Kamiyama and Yasushi Ichikawa and Koji Kadota and Rika Miki and Yasuhiro Tomaru and Yosuke Mizuno and Naoko Tominaga and Rieko Yano and Hitoshi Goto and Hiroyuki Nitanda and Shinji Togo and Yasushi Okazaki and Yoshihide Hayashizaki and Hiroshi Shimada",

note = "Funding Information: This study has been supported by Special Coordination Funds for promoting Science and Technology and a Research Grant for the RIKEN Genome Exploration Research Project fl-om the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government, Special Coordination Funds for Promoting the Biore-source Program from RIKEN Tsukuba and CREST (Core Research for Evolutional Science and Technology) and ACT-JST (Research and Development for Applying Advanced Computational Science and Technology) of Japan Science and Technology Corporation (JST). This work was also supported by a Grant-in-Aid for Scientific Research on Priority Areas and Human Genome Program, from the Ministry of Education, Science and Culture, and by a Grant-in-Aid for a Second Term Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health, Labour and Welfare to Y. H. This study was supported in part by Special Coordination Funds for Promoting Science and Technology from the Science and Technology Agency of the Japanese Government to YO.",

year = "2002",

month = jan,

doi = "10.1007/BF02967543",

language = "English",

volume = "9",

pages = "26--32",

journal = "Breast Cancer",

issn = "1340-6868",

publisher = "Springer Japan",

number = "1",

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TY - JOUR

T1 - Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray

AU - Nishizuka, Itaru

AU - Ishikawa, Takashi

AU - Hamaguchi, Yohei

AU - Kamiyama, Masako

AU - Ichikawa, Yasushi

AU - Kadota, Koji

AU - Miki, Rika

AU - Tomaru, Yasuhiro

AU - Mizuno, Yosuke

AU - Tominaga, Naoko

AU - Yano, Rieko

AU - Goto, Hitoshi

AU - Nitanda, Hiroyuki

AU - Togo, Shinji

AU - Okazaki, Yasushi

AU - Hayashizaki, Yoshihide

AU - Shimada, Hiroshi

N1 - Funding Information: This study has been supported by Special Coordination Funds for promoting Science and Technology and a Research Grant for the RIKEN Genome Exploration Research Project fl-om the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government, Special Coordination Funds for Promoting the Biore-source Program from RIKEN Tsukuba and CREST (Core Research for Evolutional Science and Technology) and ACT-JST (Research and Development for Applying Advanced Computational Science and Technology) of Japan Science and Technology Corporation (JST). This work was also supported by a Grant-in-Aid for Scientific Research on Priority Areas and Human Genome Program, from the Ministry of Education, Science and Culture, and by a Grant-in-Aid for a Second Term Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health, Labour and Welfare to Y. H. This study was supported in part by Special Coordination Funds for Promoting Science and Technology from the Science and Technology Agency of the Japanese Government to YO.

PY - 2002/1

Y1 - 2002/1

N2 - Background: Brain metastases occur in 15% to 30% of breast cancer patients, usually as a late event. The patterns of metastases to different organs are determined by the tumor cell phenotype and interactions between the tumor cells and the organ environment. Methods: We investigated the gene expression profile occurring in brain metastases from a breast cancer cell line. We used cDNA microarrays to compare patterns of gene expression between the mouse breast cancer cell line Jyg MC (A) and a subline that often metastasis to brain, (B). Results: By Microarray analysis about 350 of 21,000 genes were significantly up-regulated in Jyg MC (B). Many candidate genes that may be associated with the establishment of brain metastasis from breast cancer were included. Interestingly, we found that the expression of astrocyte derived cytokine receptors (IL-6 receptor, TGF-beta receptor and IGF receptor) were significantly increased in Jyg MC (B) cells. These results were confirmed by RT-PCR. Conclusion: These results suggest that cytokines produced by glial cells in vivo may contribute, in a paracrine manner, to the development of brain metastases from breast cancer cells.

AB - Background: Brain metastases occur in 15% to 30% of breast cancer patients, usually as a late event. The patterns of metastases to different organs are determined by the tumor cell phenotype and interactions between the tumor cells and the organ environment. Methods: We investigated the gene expression profile occurring in brain metastases from a breast cancer cell line. We used cDNA microarrays to compare patterns of gene expression between the mouse breast cancer cell line Jyg MC (A) and a subline that often metastasis to brain, (B). Results: By Microarray analysis about 350 of 21,000 genes were significantly up-regulated in Jyg MC (B). Many candidate genes that may be associated with the establishment of brain metastasis from breast cancer were included. Interestingly, we found that the expression of astrocyte derived cytokine receptors (IL-6 receptor, TGF-beta receptor and IGF receptor) were significantly increased in Jyg MC (B) cells. These results were confirmed by RT-PCR. Conclusion: These results suggest that cytokines produced by glial cells in vivo may contribute, in a paracrine manner, to the development of brain metastases from breast cancer cells.

KW - Brain metastasis

KW - Breast cancer

KW - CDNA microarray

KW - Cell line

KW - Cytokine

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U2 - 10.1007/BF02967543

DO - 10.1007/BF02967543

M3 - Article

C2 - 12196718

AN - SCOPUS:0036049011

SN - 1340-6868

VL - 9

SP - 26

EP - 32

JO - Breast Cancer

JF - Breast Cancer

IS - 1

ER -

Analysis of gene expression involved in brain metastasis from breast cancer using cDNA microarray

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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