Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography

Koji Muramoto; Rina Goto; Hisao Kamiya

doi:10.1016/0003-2697(87)90416-7

Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography

Koji Muramoto, Rina Goto, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A chromophoric hydrazide, 4′-N,N-dimethylamino-4-azobenzene sulfonyl hydrazide (DABS-hydrazide), was prepared from 4′-N,N-dimethylamino-4-azobenzene sulfonyl chloride by reaction with hydrazine. Reducing sugars were derivatized with DABS-hydrazide at 50°C for 120 min. The chromophoric hydrazones were separated by reversed-phase HPLC isocratically using a short column (4.6 × 50 mm) and 0.08 m acetic acid-acetone as an eluant with no sample pretreatment and were quantitated at the picomole level. This method was applied to the sugar analysis of 5 μg of glycoproteins. Dansyl hydrazide derivatives of sugars were also separated by this HPLC system.

Original language	English
Pages (from-to)	435-442
Number of pages	8
Journal	Analytical Biochemistry
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(87)90416-7
Publication status	Published - 1987 May 1

Keywords

HPLC
glycoproteins
sugar analysis
sugars

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(87)90416-7

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title = "Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography",

abstract = "A chromophoric hydrazide, 4′-N,N-dimethylamino-4-azobenzene sulfonyl hydrazide (DABS-hydrazide), was prepared from 4′-N,N-dimethylamino-4-azobenzene sulfonyl chloride by reaction with hydrazine. Reducing sugars were derivatized with DABS-hydrazide at 50°C for 120 min. The chromophoric hydrazones were separated by reversed-phase HPLC isocratically using a short column (4.6 × 50 mm) and 0.08 m acetic acid-acetone as an eluant with no sample pretreatment and were quantitated at the picomole level. This method was applied to the sugar analysis of 5 μg of glycoproteins. Dansyl hydrazide derivatives of sugars were also separated by this HPLC system.",

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AU - Goto, Rina

AU - Kamiya, Hisao

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Y1 - 1987/5/1

N2 - A chromophoric hydrazide, 4′-N,N-dimethylamino-4-azobenzene sulfonyl hydrazide (DABS-hydrazide), was prepared from 4′-N,N-dimethylamino-4-azobenzene sulfonyl chloride by reaction with hydrazine. Reducing sugars were derivatized with DABS-hydrazide at 50°C for 120 min. The chromophoric hydrazones were separated by reversed-phase HPLC isocratically using a short column (4.6 × 50 mm) and 0.08 m acetic acid-acetone as an eluant with no sample pretreatment and were quantitated at the picomole level. This method was applied to the sugar analysis of 5 μg of glycoproteins. Dansyl hydrazide derivatives of sugars were also separated by this HPLC system.

AB - A chromophoric hydrazide, 4′-N,N-dimethylamino-4-azobenzene sulfonyl hydrazide (DABS-hydrazide), was prepared from 4′-N,N-dimethylamino-4-azobenzene sulfonyl chloride by reaction with hydrazine. Reducing sugars were derivatized with DABS-hydrazide at 50°C for 120 min. The chromophoric hydrazones were separated by reversed-phase HPLC isocratically using a short column (4.6 × 50 mm) and 0.08 m acetic acid-acetone as an eluant with no sample pretreatment and were quantitated at the picomole level. This method was applied to the sugar analysis of 5 μg of glycoproteins. Dansyl hydrazide derivatives of sugars were also separated by this HPLC system.

KW - HPLC

KW - glycoproteins

KW - sugar analysis

KW - sugars

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JO - Analytical Biochemistry

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Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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