Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies

Masahiro Sakaguchi; Ken ich Masuda; Masako Toda; Sakae Inouye; Hiroshi Yasueda; Yoshifumi Taniguchi; Takao Nagoya; Douglas J. DeBoer; Hajime Tsujimoto

doi:10.1016/S0165-2427(00)00250-6

Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies

Masahiro Sakaguchi, Ken ich Masuda, Masako Toda, Sakae Inouye, Hiroshi Yasueda, Yoshifumi Taniguchi, Takao Nagoya, Douglas J. DeBoer, Hajime Tsujimoto

AGR - Agricultural Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In our previous study [Immunology 91 (1997) 161] using monoclonal antibodies (mAbs) specific to Cry j 1, a major allergen in Japanese cedar (Cryptomeria japonica) pollen, we identified five independent epitopes (EP-1-EP-5) on the molecule and found that EP-1 and EP-5 are the predominant allergic epitopes for humans and monkeys, respectively. In this study, we analyzed the epitopes recognized by IgE in the sera of 10 dogs sensitive to C. japonica pollen allergen using an IgE-ELISA inhibition method with these mAbs. The IgE reaction patterns varied among dogs. In eight of the 10 dogs, IgE recognized EP-5 which is a predominant allergic epitope for monkeys with the pollenosis. In four dogs, IgE recognized EP-1 which is a predominant allergic epitope for human patients with the pollenosis. In three dogs, IgE recognized EP-4 which is a heat-stable epitope. EP-5 is a predominant allergic epitope for dogs and some, but not all, dogs have IgE reaction patterns to the epitopes similar to those of humans.

Original language	English
Pages (from-to)	35-43
Number of pages	9
Journal	Veterinary Immunology and Immunopathology
Volume	78
Issue number	1
DOIs	https://doi.org/10.1016/S0165-2427(00)00250-6
Publication status	Published - 2001 Jan 10

Keywords

Allergen
Dog
Epitope
IgE
Japanese cedar pollen

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10.1016/S0165-2427(00)00250-6

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Sakaguchi, M., Masuda, K. I., Toda, M., Inouye, S., Yasueda, H., Taniguchi, Y., Nagoya, T., DeBoer, D. J., & Tsujimoto, H. (2001). Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies. Veterinary Immunology and Immunopathology, 78(1), 35-43. https://doi.org/10.1016/S0165-2427(00)00250-6

@article{674329c9952a4b90aa2f284679cc710c,

title = "Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies",

abstract = "In our previous study [Immunology 91 (1997) 161] using monoclonal antibodies (mAbs) specific to Cry j 1, a major allergen in Japanese cedar (Cryptomeria japonica) pollen, we identified five independent epitopes (EP-1-EP-5) on the molecule and found that EP-1 and EP-5 are the predominant allergic epitopes for humans and monkeys, respectively. In this study, we analyzed the epitopes recognized by IgE in the sera of 10 dogs sensitive to C. japonica pollen allergen using an IgE-ELISA inhibition method with these mAbs. The IgE reaction patterns varied among dogs. In eight of the 10 dogs, IgE recognized EP-5 which is a predominant allergic epitope for monkeys with the pollenosis. In four dogs, IgE recognized EP-1 which is a predominant allergic epitope for human patients with the pollenosis. In three dogs, IgE recognized EP-4 which is a heat-stable epitope. EP-5 is a predominant allergic epitope for dogs and some, but not all, dogs have IgE reaction patterns to the epitopes similar to those of humans.",

keywords = "Allergen, Dog, Epitope, IgE, Japanese cedar pollen",

author = "Masahiro Sakaguchi and Masuda, {Ken ich} and Masako Toda and Sakae Inouye and Hiroshi Yasueda and Yoshifumi Taniguchi and Takao Nagoya and DeBoer, {Douglas J.} and Hajime Tsujimoto",

note = "Funding Information: This work was supported by a grant from the Agency for Science and Technology, Japan.",

year = "2001",

month = jan,

day = "10",

doi = "10.1016/S0165-2427(00)00250-6",

language = "English",

volume = "78",

pages = "35--43",

journal = "Veterinary Immunology and Immunopathology",

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Sakaguchi, M, Masuda, KI, Toda, M, Inouye, S, Yasueda, H, Taniguchi, Y, Nagoya, T, DeBoer, DJ & Tsujimoto, H 2001, 'Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies', Veterinary Immunology and Immunopathology, vol. 78, no. 1, pp. 35-43. https://doi.org/10.1016/S0165-2427(00)00250-6

TY - JOUR

T1 - Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies

AU - Sakaguchi, Masahiro

AU - Masuda, Ken ich

AU - Toda, Masako

AU - Inouye, Sakae

AU - Yasueda, Hiroshi

AU - Taniguchi, Yoshifumi

AU - Nagoya, Takao

AU - DeBoer, Douglas J.

AU - Tsujimoto, Hajime

N1 - Funding Information: This work was supported by a grant from the Agency for Science and Technology, Japan.

PY - 2001/1/10

Y1 - 2001/1/10

N2 - In our previous study [Immunology 91 (1997) 161] using monoclonal antibodies (mAbs) specific to Cry j 1, a major allergen in Japanese cedar (Cryptomeria japonica) pollen, we identified five independent epitopes (EP-1-EP-5) on the molecule and found that EP-1 and EP-5 are the predominant allergic epitopes for humans and monkeys, respectively. In this study, we analyzed the epitopes recognized by IgE in the sera of 10 dogs sensitive to C. japonica pollen allergen using an IgE-ELISA inhibition method with these mAbs. The IgE reaction patterns varied among dogs. In eight of the 10 dogs, IgE recognized EP-5 which is a predominant allergic epitope for monkeys with the pollenosis. In four dogs, IgE recognized EP-1 which is a predominant allergic epitope for human patients with the pollenosis. In three dogs, IgE recognized EP-4 which is a heat-stable epitope. EP-5 is a predominant allergic epitope for dogs and some, but not all, dogs have IgE reaction patterns to the epitopes similar to those of humans.

AB - In our previous study [Immunology 91 (1997) 161] using monoclonal antibodies (mAbs) specific to Cry j 1, a major allergen in Japanese cedar (Cryptomeria japonica) pollen, we identified five independent epitopes (EP-1-EP-5) on the molecule and found that EP-1 and EP-5 are the predominant allergic epitopes for humans and monkeys, respectively. In this study, we analyzed the epitopes recognized by IgE in the sera of 10 dogs sensitive to C. japonica pollen allergen using an IgE-ELISA inhibition method with these mAbs. The IgE reaction patterns varied among dogs. In eight of the 10 dogs, IgE recognized EP-5 which is a predominant allergic epitope for monkeys with the pollenosis. In four dogs, IgE recognized EP-1 which is a predominant allergic epitope for human patients with the pollenosis. In three dogs, IgE recognized EP-4 which is a heat-stable epitope. EP-5 is a predominant allergic epitope for dogs and some, but not all, dogs have IgE reaction patterns to the epitopes similar to those of humans.

KW - Allergen

KW - Dog

KW - Epitope

KW - IgE

KW - Japanese cedar pollen

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DO - 10.1016/S0165-2427(00)00250-6

M3 - Article

C2 - 11182146

AN - SCOPUS:0035834950

SN - 0165-2427

VL - 78

SP - 35

EP - 43

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1

ER -

Analysis of the canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j 1 monoclonal antibodies

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Keywords

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