Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis

Jun Inoue; Yoshiyuki Ueno; Takayuki Kogure; Futoshi Nagasaki; Osamu Kimura; Noriyuki Obara; Osamu Kido; Yu Nakagome; Eiji Kakazu; Yasunori Matsuda; Koji Fukushima; Haruna Segawa; Ichiro Nakajima; Yasuto Itoyama; Masaharu Takahashi; Hiroaki Okamoto; Tooru Shimosegawa

doi:10.1016/j.jcv.2008.01.002

Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis

Jun Inoue, Yoshiyuki Ueno, Takayuki Kogure, Futoshi Nagasaki, Osamu Kimura, Noriyuki Obara, Osamu Kido, Yu Nakagome, Eiji Kakazu, Yasunori Matsuda, Koji Fukushima, Haruna Segawa, Ichiro Nakajima, Yasuto Itoyama, Masaharu Takahashi, Hiroaki Okamoto, Tooru Shimosegawa

HOSP - Internal Medicine

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Although many extrahepatic manifestations have been described in patients with acute or chronic hepatitis B, there are few reports about neurological disorders. We describe a 55-year-old man who contracted acute hepatitis B virus (HBV) infection and transverse myelitis. His neurological findings were gradually reduced along with the recovery from hepatitis. The cerebrospinal fluid (CSF) was revealed to be positive for HBsAg and HBV DNA. Full-length sequences of HBV in his serum and CSF were determined, and it was revealed that these two isolates had mutations at nucleotide (nt) 1762/1764 in the core promoter region and nt 1896 in the precore region. They were identical to each other except for two ambiguous codes at nt 2020 and 2631 in the CSF isolate. After cloning of the amplicons, substitutions at nt 2020 and 2631 were found in 6 (38%) of the 16 CSF clones. One clone of the 6 CSF clones had an additional substitution at nt 2119. These substitutions were not found in 16 serum clones. The presence of HBV clones unique to CSF suggests that HBV was a possible causative agent of the myelitis.

Original language	English
Pages (from-to)	301-304
Number of pages	4
Journal	Journal of Clinical Virology
Volume	41
Issue number	4
DOIs	https://doi.org/10.1016/j.jcv.2008.01.002
Publication status	Published - 2008 Apr

Keywords

Acute hepatitis B
Cerebrospinal fluid
Full-length genome
Hepatitis B virus
Sequence analysis
Transverse myelitis

UN SDGs

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10.1016/j.jcv.2008.01.002

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Inoue, J., Ueno, Y., Kogure, T., Nagasaki, F., Kimura, O., Obara, N., Kido, O., Nakagome, Y., Kakazu, E., Matsuda, Y., Fukushima, K., Segawa, H., Nakajima, I., Itoyama, Y., Takahashi, M., Okamoto, H., & Shimosegawa, T. (2008). Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis. Journal of Clinical Virology, 41(4), 301-304. https://doi.org/10.1016/j.jcv.2008.01.002

@article{db35465a699c4545954a0d18693ab1ef,

title = "Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis",

abstract = "Although many extrahepatic manifestations have been described in patients with acute or chronic hepatitis B, there are few reports about neurological disorders. We describe a 55-year-old man who contracted acute hepatitis B virus (HBV) infection and transverse myelitis. His neurological findings were gradually reduced along with the recovery from hepatitis. The cerebrospinal fluid (CSF) was revealed to be positive for HBsAg and HBV DNA. Full-length sequences of HBV in his serum and CSF were determined, and it was revealed that these two isolates had mutations at nucleotide (nt) 1762/1764 in the core promoter region and nt 1896 in the precore region. They were identical to each other except for two ambiguous codes at nt 2020 and 2631 in the CSF isolate. After cloning of the amplicons, substitutions at nt 2020 and 2631 were found in 6 (38%) of the 16 CSF clones. One clone of the 6 CSF clones had an additional substitution at nt 2119. These substitutions were not found in 16 serum clones. The presence of HBV clones unique to CSF suggests that HBV was a possible causative agent of the myelitis.",

keywords = "Acute hepatitis B, Cerebrospinal fluid, Full-length genome, Hepatitis B virus, Sequence analysis, Transverse myelitis",

author = "Jun Inoue and Yoshiyuki Ueno and Takayuki Kogure and Futoshi Nagasaki and Osamu Kimura and Noriyuki Obara and Osamu Kido and Yu Nakagome and Eiji Kakazu and Yasunori Matsuda and Koji Fukushima and Haruna Segawa and Ichiro Nakajima and Yasuto Itoyama and Masaharu Takahashi and Hiroaki Okamoto and Tooru Shimosegawa",

note = "Funding Information: This work was supported by Health and Labour Sciences Research Grants (from the Ministry of Health, Labour and Welfare of Japan) for the Research on Measures for Intractable Diseases.",

year = "2008",

month = apr,

doi = "10.1016/j.jcv.2008.01.002",

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Inoue, J, Ueno, Y, Kogure, T, Nagasaki, F, Kimura, O, Obara, N, Kido, O, Nakagome, Y, Kakazu, E, Matsuda, Y, Fukushima, K, Segawa, H, Nakajima, I, Itoyama, Y, Takahashi, M, Okamoto, H & Shimosegawa, T 2008, 'Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis', Journal of Clinical Virology, vol. 41, no. 4, pp. 301-304. https://doi.org/10.1016/j.jcv.2008.01.002

TY - JOUR

T1 - Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis

AU - Inoue, Jun

AU - Ueno, Yoshiyuki

AU - Kogure, Takayuki

AU - Nagasaki, Futoshi

AU - Kimura, Osamu

AU - Obara, Noriyuki

AU - Kido, Osamu

AU - Nakagome, Yu

AU - Kakazu, Eiji

AU - Matsuda, Yasunori

AU - Fukushima, Koji

AU - Segawa, Haruna

AU - Nakajima, Ichiro

AU - Itoyama, Yasuto

AU - Takahashi, Masaharu

AU - Okamoto, Hiroaki

AU - Shimosegawa, Tooru

N1 - Funding Information: This work was supported by Health and Labour Sciences Research Grants (from the Ministry of Health, Labour and Welfare of Japan) for the Research on Measures for Intractable Diseases.

PY - 2008/4

Y1 - 2008/4

N2 - Although many extrahepatic manifestations have been described in patients with acute or chronic hepatitis B, there are few reports about neurological disorders. We describe a 55-year-old man who contracted acute hepatitis B virus (HBV) infection and transverse myelitis. His neurological findings were gradually reduced along with the recovery from hepatitis. The cerebrospinal fluid (CSF) was revealed to be positive for HBsAg and HBV DNA. Full-length sequences of HBV in his serum and CSF were determined, and it was revealed that these two isolates had mutations at nucleotide (nt) 1762/1764 in the core promoter region and nt 1896 in the precore region. They were identical to each other except for two ambiguous codes at nt 2020 and 2631 in the CSF isolate. After cloning of the amplicons, substitutions at nt 2020 and 2631 were found in 6 (38%) of the 16 CSF clones. One clone of the 6 CSF clones had an additional substitution at nt 2119. These substitutions were not found in 16 serum clones. The presence of HBV clones unique to CSF suggests that HBV was a possible causative agent of the myelitis.

AB - Although many extrahepatic manifestations have been described in patients with acute or chronic hepatitis B, there are few reports about neurological disorders. We describe a 55-year-old man who contracted acute hepatitis B virus (HBV) infection and transverse myelitis. His neurological findings were gradually reduced along with the recovery from hepatitis. The cerebrospinal fluid (CSF) was revealed to be positive for HBsAg and HBV DNA. Full-length sequences of HBV in his serum and CSF were determined, and it was revealed that these two isolates had mutations at nucleotide (nt) 1762/1764 in the core promoter region and nt 1896 in the precore region. They were identical to each other except for two ambiguous codes at nt 2020 and 2631 in the CSF isolate. After cloning of the amplicons, substitutions at nt 2020 and 2631 were found in 6 (38%) of the 16 CSF clones. One clone of the 6 CSF clones had an additional substitution at nt 2119. These substitutions were not found in 16 serum clones. The presence of HBV clones unique to CSF suggests that HBV was a possible causative agent of the myelitis.

KW - Acute hepatitis B

KW - Cerebrospinal fluid

KW - Full-length genome

KW - Hepatitis B virus

KW - Sequence analysis

KW - Transverse myelitis

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U2 - 10.1016/j.jcv.2008.01.002

DO - 10.1016/j.jcv.2008.01.002

M3 - Article

C2 - 18291715

AN - SCOPUS:40849099839

SN - 1386-6532

VL - 41

SP - 301

EP - 304

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

IS - 4

ER -

Analysis of the full-length genome of hepatitis B virus in the serum and cerebrospinal fluid of a patient with acute hepatitis B and transverse myelitis

Abstract

Keywords

UN SDGs

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