Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

Koji Muramoto; Tomoko Kuriki; Kiyoshi Nokihara; Hiroshi Yako; Hisao Kamiya

doi:10.2331/fishsci.62.435

Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

Koji Muramoto, Tomoko Kuriki, Kiyoshi Nokihara, Hiroshi Yako, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

Abstract

The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl: 4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.

Original language	English
Pages (from-to)	435-438
Number of pages	4
Journal	Fisheries Science
Volume	62
Issue number	3
DOIs	https://doi.org/10.2331/fishsci.62.435
Publication status	Published - 1996 Jun

Keywords

Amino acid sequence
Barnacle
Hemolymph protein
Lectins
Two-dimensional gel electrophoresis

ASJC Scopus subject areas

Aquatic Science

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10.2331/fishsci.62.435

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title = "Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis",

abstract = "The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl: 4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.",

keywords = "Amino acid sequence, Barnacle, Hemolymph protein, Lectins, Two-dimensional gel electrophoresis",

author = "Koji Muramoto and Tomoko Kuriki and Kiyoshi Nokihara and Hiroshi Yako and Hisao Kamiya",

year = "1996",

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AU - Muramoto, Koji

AU - Kuriki, Tomoko

AU - Nokihara, Kiyoshi

AU - Yako, Hiroshi

AU - Kamiya, Hisao

PY - 1996/6

Y1 - 1996/6

N2 - The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl: 4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.

AB - The hemolymph proteins of the acorn barnacle Megabalanus rosa were separated by two-dimensional gel electrophoresis. More than 50 proteins were observed in the region of acidic (pl: 4-7) with masses of 18-120 kDa. The proteins separated on the gel were electroblotted onto a polyvinylidene difluoride membrane and were sequenced. Several major proteins were further characterized by amino acid analysis and peptide mapping, and identified to be multiple lectins.

KW - Amino acid sequence

KW - Barnacle

KW - Hemolymph protein

KW - Lectins

KW - Two-dimensional gel electrophoresis

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Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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