Anion sensor-based ratiometric peptide probe for protein kinase activity

Kazuya Kikuchi, Shigeki Hashimoto, Shin Mizukami, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


A new fluorescent sensor consisting of Cd"-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which In turn displaces the coumarin fluorophore, and this event results In ratiometric change of excitation spectrum In neutral aqueous solution.

Original languageEnglish
Pages (from-to)2732-2735
Number of pages4
JournalOrganic letters
Issue number13
Publication statusPublished - 2009 Jul 2
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


Dive into the research topics of 'Anion sensor-based ratiometric peptide probe for protein kinase activity'. Together they form a unique fingerprint.

Cite this