Anion sensor-based ratiometric peptide probe for protein kinase activity

Kazuya Kikuchi; Shigeki Hashimoto; Shin Mizukami; Tetsuo Nagano

doi:10.1021/ol9006508

Anion sensor-based ratiometric peptide probe for protein kinase activity

Kazuya Kikuchi, Shigeki Hashimoto, Shin Mizukami, Tetsuo Nagano

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

A new fluorescent sensor consisting of Cd"-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which In turn displaces the coumarin fluorophore, and this event results In ratiometric change of excitation spectrum In neutral aqueous solution.

Original language	English
Pages (from-to)	2732-2735
Number of pages	4
Journal	Organic letters
Volume	11
Issue number	13
DOIs	https://doi.org/10.1021/ol9006508
Publication status	Published - 2009 Jul 2
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol9006508

Cite this

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abstract = "A new fluorescent sensor consisting of Cd{"}-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which In turn displaces the coumarin fluorophore, and this event results In ratiometric change of excitation spectrum In neutral aqueous solution.",

author = "Kazuya Kikuchi and Shigeki Hashimoto and Shin Mizukami and Tetsuo Nagano",

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AU - Kikuchi, Kazuya

AU - Hashimoto, Shigeki

AU - Mizukami, Shin

AU - Nagano, Tetsuo

PY - 2009/7/2

Y1 - 2009/7/2

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AB - A new fluorescent sensor consisting of Cd"-cylcen appended aminocoumarin and a substrate peptide for protein kinase A (PKA) has been designed. Upon phosphorylation by PKA, the metal complex moiety binds to a phosphorylated residue, which In turn displaces the coumarin fluorophore, and this event results In ratiometric change of excitation spectrum In neutral aqueous solution.

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Anion sensor-based ratiometric peptide probe for protein kinase activity

Abstract

ASJC Scopus subject areas

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