Anti-angiogenic effects of conjugated docosahexaenoic acid in vitro and in vivo

Tsuyoshi Tsuzuki, Akira Shibata, Yuki Kawakami, Kiyotaka Nakagaya, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


The anti-angiogenic effects of conjugated docosahexaenoic acid (CDHA), which was prepared by an alkaline treatment of docosahexaenoic acid and contained conjugated double bonds, were investigated in vitro and in vivo. CDHA inhibited tube formation by the bovine aortic endothelial cell (BAEC), and also inhibited the proliferation of BAEC at a concentration of CDHA that suppressed tube formation, but did not influence cell migration. The inhibition of BAEC growth caused by CDHA was accompanied by a marked change in cellular morphology. Nuclear condensation and brightness were observed in Hoechst 33342-stained cells treated with CDHA, indicating that CDHA induced apoptosis in BAEC. We also evaluated the angiogenesis inhibition of CDHA in vivo. The vessel formation which was triggered by tumor cells was clearly suppressed in mice orally given CDHA. Our findings suggest that CDHA has potential use as a therapeutic dietary supplement for minimizing tumor angiogenesis.

Original languageEnglish
Pages (from-to)1902-1910
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Issue number8
Publication statusPublished - 2007


  • Angiogenesis
  • Anti-angiogenic effect
  • Apoptosis
  • Conjugated docosahexaenoic acid
  • Conjugated fatty acid


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