Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

Shinji Yamada; Mika K. Kaneko; Yoshikazu Furusawa; Shunsuke Itai; Masato Sano; Takuro Nakamura; Miyuki Yanaka; Saori Handa; Kayo Hisamatsu; Yoshimi Nakamura; Masanori Koyanagi; Masato Fukui; Hiroyuki Harada; Yukinari Kato

doi:10.1089/mab.2018.0031

Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

Shinji Yamada, Mika K. Kaneko, Yoshikazu Furusawa, Shunsuke Itai, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Kayo Hisamatsu, Yoshimi Nakamura, Masanori Koyanagi, Masato Fukui, Hiroyuki Harada, Yukinari Kato

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG₁, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.

Original language	English
Pages (from-to)	96-99
Number of pages	4
Journal	Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume	38
Issue number	2
DOIs	https://doi.org/10.1089/mab.2018.0031
Publication status	Published - 2019 Apr 1

Keywords

goat podoplanin
PDPN
PMab-44

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10.1089/mab.2018.0031

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Yamada, S., Kaneko, M. K., Furusawa, Y., Itai, S., Sano, M., Nakamura, T., Yanaka, M., Handa, S., Hisamatsu, K., Nakamura, Y., Koyanagi, M., Fukui, M., Harada, H., & Kato, Y. (2019). Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 38(2), 96-99. https://doi.org/10.1089/mab.2018.0031

@article{6476978cc0854323bc511afa5d77972f,

title = "Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry",

abstract = "Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.",

keywords = "goat podoplanin, PDPN, PMab-44",

author = "Shinji Yamada and Kaneko, {Mika K.} and Yoshikazu Furusawa and Shunsuke Itai and Masato Sano and Takuro Nakamura and Miyuki Yanaka and Saori Handa and Kayo Hisamatsu and Yoshimi Nakamura and Masanori Koyanagi and Masato Fukui and Hiroyuki Harada and Yukinari Kato",

note = "Funding Information: This research was supported in part by AMED under grant numbers JP18am0101078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI grant numbers 17K07299 (M.K.K.) and 16K10748 (Y.K.). Publisher Copyright: {\textcopyright} 2019, Mary Ann Liebert, Inc.",

year = "2019",

month = apr,

day = "1",

doi = "10.1089/mab.2018.0031",

language = "English",

volume = "38",

pages = "96--99",

journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",

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publisher = "Mary Ann Liebert Inc.",

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Yamada, S, Kaneko, MK, Furusawa, Y, Itai, S, Sano, M, Nakamura, T, Yanaka, M, Handa, S, Hisamatsu, K, Nakamura, Y, Koyanagi, M, Fukui, M, Harada, H & Kato, Y 2019, 'Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry', Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, vol. 38, no. 2, pp. 96-99. https://doi.org/10.1089/mab.2018.0031

TY - JOUR

T1 - Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

AU - Yamada, Shinji

AU - Kaneko, Mika K.

AU - Furusawa, Yoshikazu

AU - Itai, Shunsuke

AU - Sano, Masato

AU - Nakamura, Takuro

AU - Yanaka, Miyuki

AU - Handa, Saori

AU - Hisamatsu, Kayo

AU - Nakamura, Yoshimi

AU - Koyanagi, Masanori

AU - Fukui, Masato

AU - Harada, Hiroyuki

AU - Kato, Yukinari

N1 - Funding Information: This research was supported in part by AMED under grant numbers JP18am0101078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI grant numbers 17K07299 (M.K.K.) and 16K10748 (Y.K.). Publisher Copyright: © 2019, Mary Ann Liebert, Inc.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.

AB - Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.

KW - goat podoplanin

KW - PDPN

KW - PMab-44

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SN - 2167-9436

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JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

IS - 2

ER -

Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

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Keywords

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