TY - JOUR
T1 - Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel α 1 subunits in the dorsal horn of spinal cord in mice
AU - Murakami, Manabu
AU - Nakagawasai, Osamu
AU - Suzuki, Takashi
AU - Mobarakeh, Izadi I.
AU - Sakurada, Yumiko
AU - Murata, Astunobu
AU - Yamadera, Fumihiro
AU - Miyoshi, Ichiro
AU - Yanai, Kazuhiko
AU - Tan-No, Koichi
AU - Sasano, Hironobu
AU - Tadano, Takeshi
AU - Iijima, Toshihiko
N1 - Funding Information:
We thank Kumiko Hidaka for technical assistance and Dr. Kyoichi Ono for technical advice. This research was sponsored by grants-in-aid from the Ministry of Education, Science, and Culture of Japan.
PY - 2004/10/22
Y1 - 2004/10/22
N2 - To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of ω-agatoxin TK, ω-agatoxin IVA, ω-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord.
AB - To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of ω-agatoxin TK, ω-agatoxin IVA, ω-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord.
KW - Calcium channel
KW - Mouse
KW - Nociception
KW - SNX-482
KW - Toxin
KW - ω-Agatoxin TK
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U2 - 10.1016/j.brainres.2004.07.066
DO - 10.1016/j.brainres.2004.07.066
M3 - Article
C2 - 15451373
AN - SCOPUS:4644350854
SN - 0006-8993
VL - 1024
SP - 122
EP - 129
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -