TY - JOUR
T1 - Antioxidant properties of tripeptides revealed by a comparison of six different assays
AU - Ohashi, Yumi
AU - Onuma, Ryo
AU - Naganuma, Takako
AU - Ogawa, Tomohisa
AU - Naude, Ryno
AU - Nokihara, Kiyoshi
AU - Muramoto, Koji
N1 - Publisher Copyright:
© 2015, Japanese Society for Food Science and Technology.
PY - 2015
Y1 - 2015
N2 - Free radical scavenging activities of two series of tripeptide libraries were investigated using ABTS, DPPH, and ORAC assays. Two Tyr-containing tripeptides showed higher scavenging activities against the hydrophilic ABTS and AAPH radicals than two His-containing tripeptides, showing that Tyr residues play important roles in free radical scavenging activity. Higher concentrations of tripeptides were required to reveal apparent DPPH radical scavenging activity. The antioxidant activities of the tripeptide libraries obtained from this study were compared with previous results obtained using several assays, including antioxidant activity against the peroxidation of linoleic acid, FRAP assay, and peroxynitrite (PN) scavenging activity. The antioxidant activity of the tripeptides against the peroxidation of linoleic acid showed high correlations with the ABTS and ORAC assays (correlation coefficients (R) = 0.725 and 0.731, respectively), and low correlations with FRAP, PN scavenging, and DPPH assays. The highest correlation was found between the ABTS and ORAC assays (R = 0.905).
AB - Free radical scavenging activities of two series of tripeptide libraries were investigated using ABTS, DPPH, and ORAC assays. Two Tyr-containing tripeptides showed higher scavenging activities against the hydrophilic ABTS and AAPH radicals than two His-containing tripeptides, showing that Tyr residues play important roles in free radical scavenging activity. Higher concentrations of tripeptides were required to reveal apparent DPPH radical scavenging activity. The antioxidant activities of the tripeptide libraries obtained from this study were compared with previous results obtained using several assays, including antioxidant activity against the peroxidation of linoleic acid, FRAP assay, and peroxynitrite (PN) scavenging activity. The antioxidant activity of the tripeptides against the peroxidation of linoleic acid showed high correlations with the ABTS and ORAC assays (correlation coefficients (R) = 0.725 and 0.731, respectively), and low correlations with FRAP, PN scavenging, and DPPH assays. The highest correlation was found between the ABTS and ORAC assays (R = 0.905).
KW - Antioxidant peptide library
KW - Antioxidant peptides
KW - Free radical scavenging activity
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U2 - 10.3136/fstr.21.695
DO - 10.3136/fstr.21.695
M3 - Article
AN - SCOPUS:84948734123
SN - 1344-6606
VL - 21
SP - 695
EP - 704
JO - Food Science and Technology Research
JF - Food Science and Technology Research
IS - 5
ER -