Antioxidant properties of tripeptides revealed by a comparison of six different assays

Yumi Ohashi, Ryo Onuma, Takako Naganuma, Tomohisa Ogawa, Ryno Naude, Kiyoshi Nokihara, Koji Muramoto

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Free radical scavenging activities of two series of tripeptide libraries were investigated using ABTS, DPPH, and ORAC assays. Two Tyr-containing tripeptides showed higher scavenging activities against the hydrophilic ABTS and AAPH radicals than two His-containing tripeptides, showing that Tyr residues play important roles in free radical scavenging activity. Higher concentrations of tripeptides were required to reveal apparent DPPH radical scavenging activity. The antioxidant activities of the tripeptide libraries obtained from this study were compared with previous results obtained using several assays, including antioxidant activity against the peroxidation of linoleic acid, FRAP assay, and peroxynitrite (PN) scavenging activity. The antioxidant activity of the tripeptides against the peroxidation of linoleic acid showed high correlations with the ABTS and ORAC assays (correlation coefficients (R) = 0.725 and 0.731, respectively), and low correlations with FRAP, PN scavenging, and DPPH assays. The highest correlation was found between the ABTS and ORAC assays (R = 0.905).

Original languageEnglish
Pages (from-to)695-704
Number of pages10
JournalFood Science and Technology Research
Issue number5
Publication statusPublished - 2015


  • Antioxidant peptide library
  • Antioxidant peptides
  • Free radical scavenging activity


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