TY - JOUR
T1 - Apoptosis by RGD-containing peptides observed in hemocytes of the Pacific oyster, Crassostrea gigas
AU - Terahara, Kazutaka
AU - Takahashi, Keisuke G.
AU - Mori, Katsuyoshi
N1 - Funding Information:
We thank the staff of the Education and Research Center of Marine Biosources at Onagawa, Tohoku University, for their help in our experiments. We are also grateful to Dr Kerry Greer for reading and correcting the manuscript. This study was supported in part by a Grant-in-Aid for scientific research (13660176) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2003/6
Y1 - 2003/6
N2 - We observed in vitro that after treatment with the Arg-Gly-Asp (RGD) peptide, non-spreading Crassostrea gigas hemocytes underwent cell death. Utilizing a combination of a Hoechst staining method and a DNA fragmentation assay, the typical features of apoptosis were shown, i.e. cell shrinkage, membrane blebbing, chromatin condensation, and DNA fragmentation. The hemocyte cell death caused by the RGD peptide appears to be sequence-specific, since no induction was shown in the alanine-substituted control peptide (RAD) treatment. Interestingly, the glutamic acid-substituted control peptide (RGE) also induced hemocytic cell death, but a different type of the death to that induced by the RGD peptide. This is the first report that specific peptides induce cell death in molluscan hemocytes.
AB - We observed in vitro that after treatment with the Arg-Gly-Asp (RGD) peptide, non-spreading Crassostrea gigas hemocytes underwent cell death. Utilizing a combination of a Hoechst staining method and a DNA fragmentation assay, the typical features of apoptosis were shown, i.e. cell shrinkage, membrane blebbing, chromatin condensation, and DNA fragmentation. The hemocyte cell death caused by the RGD peptide appears to be sequence-specific, since no induction was shown in the alanine-substituted control peptide (RAD) treatment. Interestingly, the glutamic acid-substituted control peptide (RGE) also induced hemocytic cell death, but a different type of the death to that induced by the RGD peptide. This is the first report that specific peptides induce cell death in molluscan hemocytes.
KW - Apoptosis
KW - Cell adhesion
KW - Hemocyte
KW - Integrin
KW - Oyster
KW - RGD peptide
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U2 - 10.1016/S0145-305X(02)00159-3
DO - 10.1016/S0145-305X(02)00159-3
M3 - Article
C2 - 12697309
AN - SCOPUS:0037409617
SN - 0145-305X
VL - 27
SP - 521
EP - 528
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 6-7
ER -