Application of fluoresceinisothiocyanate labeling of protein to sds-electrophoresis and subsequent sequence analysis

Koji Muramoto; Hiroshi Meguro; Katura Tuzimura

doi:10.1080/00021369.1977.10862806

Application of fluoresceinisothiocyanate labeling of protein to sds-electrophoresis and subsequent sequence analysis

Koji Muramoto, Hiroshi Meguro, Katura Tuzimura

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Fluoresceinisothiocyanate was applied to fluorescent labeling of protein for the determination of molecular weight by SDS polyacrylamide gel electrophoresis. Proteins having molecular weight ranging from 15, 000 to 70, 000 were tested. The resolved FITC-labeled protein was eluted from the gel and was submitted to amino acid sequence analysis.

Original language	English
Pages (from-to)	2059-2062
Number of pages	4
Journal	Agricultural and Biological Chemistry
Volume	41
Issue number	10
DOIs	https://doi.org/10.1080/00021369.1977.10862806
Publication status	Published - 1977 Oct

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1080/00021369.1977.10862806

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title = "Application of fluoresceinisothiocyanate labeling of protein to sds-electrophoresis and subsequent sequence analysis",

abstract = "Fluoresceinisothiocyanate was applied to fluorescent labeling of protein for the determination of molecular weight by SDS polyacrylamide gel electrophoresis. Proteins having molecular weight ranging from 15, 000 to 70, 000 were tested. The resolved FITC-labeled protein was eluted from the gel and was submitted to amino acid sequence analysis.",

author = "Koji Muramoto and Hiroshi Meguro and Katura Tuzimura",

year = "1977",

month = oct,

doi = "10.1080/00021369.1977.10862806",

language = "English",

volume = "41",

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journal = "Bioscience, Biotechnology and Biochemistry",

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number = "10",

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T1 - Application of fluoresceinisothiocyanate labeling of protein to sds-electrophoresis and subsequent sequence analysis

AU - Muramoto, Koji

AU - Meguro, Hiroshi

AU - Tuzimura, Katura

PY - 1977/10

Y1 - 1977/10

N2 - Fluoresceinisothiocyanate was applied to fluorescent labeling of protein for the determination of molecular weight by SDS polyacrylamide gel electrophoresis. Proteins having molecular weight ranging from 15, 000 to 70, 000 were tested. The resolved FITC-labeled protein was eluted from the gel and was submitted to amino acid sequence analysis.

AB - Fluoresceinisothiocyanate was applied to fluorescent labeling of protein for the determination of molecular weight by SDS polyacrylamide gel electrophoresis. Proteins having molecular weight ranging from 15, 000 to 70, 000 were tested. The resolved FITC-labeled protein was eluted from the gel and was submitted to amino acid sequence analysis.

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M3 - Article

AN - SCOPUS:84155166067

SN - 0916-8451

VL - 41

SP - 2059

EP - 2062

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 10

ER -

Application of fluoresceinisothiocyanate labeling of protein to sds-electrophoresis and subsequent sequence analysis

Abstract

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