Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

Takakazu Nakabayashi; Hui Ping Wang; Masataka Kinjo; Nobuhiro Ohta

doi:10.1039/b800391b

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

Takakazu Nakabayashi, Hui Ping Wang, Masataka Kinjo, Nobuhiro Ohta

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

Original language	English
Pages (from-to)	668-670
Number of pages	3
Journal	Photochemical and Photobiological Sciences
Volume	7
Issue number	6
DOIs	https://doi.org/10.1039/b800391b
Publication status	Published - 2008

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10.1039/b800391b

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title = "Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements",

abstract = "We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.",

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AU - Wang, Hui Ping

AU - Kinjo, Masataka

AU - Ohta, Nobuhiro

PY - 2008

Y1 - 2008

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AB - We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

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Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

Abstract

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