TY - GEN
T1 - Arrangement of cells within semi-closed space with microelectrode-based technique
AU - Hashimoto, Masahiko
AU - Sekine, Soichiro
AU - Kawashima, Takeaki
AU - Kaji, Hirokazu
AU - Nishizawa, Matsuhiko
N1 - Funding Information:
This study was supported by Industrial Technology eR search Grant Program in 05 from NEDO of Japan, and by a Grant-in-Aid for Scientific eR search B (No. 17310080) from the Ministry of Education, Science and Culture, Japan. M.H. acknowledges support from a research fellowship of the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2007 CBMS.
PY - 2007
Y1 - 2007
N2 - This paper reports strategies for the site-specific immobilization of cells within three-dimensional structures, which are based on a newly developed surface patterning technique, electrochemical bio-lithography. The technique uses a microelectrode to electrochemically generate oxidizing agent that causes local detachment of the anti-biofouling molecules from a substrate surface. Subsequent adsorption of cell-adhesive protein on the newly exposed surface induces local attachment. We combine this technique with dielectrophoresis (DEP) to rapidly collect cells on the cell-adhesive region created in a microfluidic chip. Also, the combination of the electrochemical bio-lithography with layer-by-layer deposition enabled the formation of patterned co-culthres inside elastic tubing structures.
AB - This paper reports strategies for the site-specific immobilization of cells within three-dimensional structures, which are based on a newly developed surface patterning technique, electrochemical bio-lithography. The technique uses a microelectrode to electrochemically generate oxidizing agent that causes local detachment of the anti-biofouling molecules from a substrate surface. Subsequent adsorption of cell-adhesive protein on the newly exposed surface induces local attachment. We combine this technique with dielectrophoresis (DEP) to rapidly collect cells on the cell-adhesive region created in a microfluidic chip. Also, the combination of the electrochemical bio-lithography with layer-by-layer deposition enabled the formation of patterned co-culthres inside elastic tubing structures.
KW - Cell patterning
KW - Co-cultivation
KW - Dielectrophoresis
KW - Electrochemical method
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UR - http://www.scopus.com/inward/citedby.url?scp=85088681809&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85088681809
T3 - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
SP - 1318
EP - 1320
BT - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
A2 - Viovy, Jean-Louis
A2 - Tabeling, Patrick
A2 - Descroix, Stephanie
A2 - Malaquin, Laurent
PB - Chemical and Biological Microsystems Society
T2 - 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Y2 - 7 October 2007 through 11 October 2007
ER -