Assessment of radical scavenging capacity and lipid peroxidation inhibiting capacity of antioxidant

Etsuo Niki, Yo Omata, Akiko Fukuhara, Yoshiro Saito, Yasukazu Yoshida

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


The role of radical scavenging antioxidants against oxidative stress has received much attention, and the antioxidant capacity has been assessed by various methods. Among them, a method that measures the effect of antioxidant on decay of the probe is one of the most widely used methods. The present study was performed to compare the two methods to assess the antioxidant capacity, one to follow the decay of the probe and the other to measure lipid peroxidation products in human plasma. It was shown that the method following probe decay was suitable for assessment of radical scavenging capacity of antioxidant, but not for the capacity to inhibit lipid peroxidation in plasma. This is true whether a hydrophilic or lipophilic probe is used. Such different results arise from the fact that the efficacy of inhibition of lipid peroxidation by antioxidants depends on the fate of antioxidantderived radical and interaction between antioxidants as well as the capacity of free radical scavenging. Thus, the capacity of antioxidants for inhibition of lipid peroxidation should be assessed from the effect on the extent of oxidation, not from the effect on probe decay.

Original languageEnglish
Pages (from-to)8255-8260
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number18
Publication statusPublished - 2008 Sept 24


  • Antioxidant capacity
  • Free radical
  • Lipid peroxidation
  • ORAC method


Dive into the research topics of 'Assessment of radical scavenging capacity and lipid peroxidation inhibiting capacity of antioxidant'. Together they form a unique fingerprint.

Cite this