TY - JOUR
T1 - Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity
AU - Hanbal, Sara E.
AU - Takashima, Keisuke
AU - Miyashita, Shuhei
AU - Ando, Sugihiro
AU - Ito, Kumiko
AU - Elsharkawy, Mohsen M.
AU - Kaneko, Toshiro
AU - Takahashi, Hideki
N1 - Funding Information:
Funding This study was funded by grants for “Scientific Research on Innovative Areas” from the Ministry of Education, Culture, Science, Sports and Technology (MEXT), Japan (grant numbers 16H06429, 16K21723, and 16H06435), and by the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”. This work was also supported by the Egyptian Government.
Funding Information:
for “Scientific Research on Innovative Areas” from the Ministry of Education, Culture, Science, Sports and Technology (MEXT), Japan (grant numbers 16H06429, 16K21723, and 16H06435), and by the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”. This work was also supported by the Egyptian Government.
Funding Information:
Acknowledgements This study was financially supported by grants
Publisher Copyright:
© 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Low-temperature atmospheric-pressure air plasma is a source of charged and neutral gas species. In this study, N-carrying tobacco plants were inoculated with plasma irradiated and non-irradiated tobacco mosaic virus (TMV) solution, resulting in necrotic local lesions on non-irradiated, but not on irradiated, TMV-inoculated leaves. Virus particles were disrupted by plasma irradiation in an exposure-dependent manner, but the viral coat protein subunit was not. TMV RNA was also fragmented in a time-dependent manner. These results indicate that plasma irradiation of TMV can collapse viral particles to the subunit level, degrading TMV RNA and thereby leading to a loss of infectivity.
AB - Low-temperature atmospheric-pressure air plasma is a source of charged and neutral gas species. In this study, N-carrying tobacco plants were inoculated with plasma irradiated and non-irradiated tobacco mosaic virus (TMV) solution, resulting in necrotic local lesions on non-irradiated, but not on irradiated, TMV-inoculated leaves. Virus particles were disrupted by plasma irradiation in an exposure-dependent manner, but the viral coat protein subunit was not. TMV RNA was also fragmented in a time-dependent manner. These results indicate that plasma irradiation of TMV can collapse viral particles to the subunit level, degrading TMV RNA and thereby leading to a loss of infectivity.
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U2 - 10.1007/s00705-018-3909-4
DO - 10.1007/s00705-018-3909-4
M3 - Article
C2 - 29948382
AN - SCOPUS:85048530672
SN - 0304-8608
VL - 163
SP - 2835
EP - 2840
JO - Archives of Virology
JF - Archives of Virology
IS - 10
ER -