TY - GEN
T1 - Atomic force microscopic imaging of the intracellular membrane surface of prestin-expressing Chinese hamster ovary cells
AU - Wada, H.
AU - Murakoshi, M.
AU - Iida, K.
AU - Kumano, S.
AU - Gomi, T.
AU - Kimura, K.
AU - Usukura, H.
AU - Sugawara, M.
AU - Kakehata, S.
AU - Ikeda, K.
AU - Katori, Y.
AU - Kobayashi, T.
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research on Priority Areas 15086202 from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by a Health and Labour Science Research Grant from the Ministry of Health, Labour and Welfare of Japan and by a grant from the Human Frontier Science Program.
Publisher Copyright:
Copyright © 2006 by World Scientific Publishing Co. Pte. Ltd.
PY - 2005
Y1 - 2005
N2 - The high sensitivity of human hearing is believed to be achieved by cochlear amplification. The basis of this amplification is thought to be the motility of mammalian outer hair cells (OHCs), i.e., OHCs elongate and contract in response to acoustical stimulation. This motility is made possible by both the cytoskeleton beneath the OHC plasma membrane and the motor protein prestin distributed throughout the plasma membrane. However, these factors have not yet been fully clarified. In the present study, therefore, attempts were made to observe the ultrastructure of the cytoskeleton of Guinea pig OHCs and to identify the motor protein prestin expressed in the plasma membrane of Chinese hamster ovary (CHO) cells by atomic force microscopy (AFM). Results indicate that the OHC cytoskeleton is comprised of circumferential actin filaments and spectrin cross-links and that particle-like structures with a diameter of 8–12 nm which exist in the plasma membrane of the prestin-expressing CHO cells are most likely be prestin.
AB - The high sensitivity of human hearing is believed to be achieved by cochlear amplification. The basis of this amplification is thought to be the motility of mammalian outer hair cells (OHCs), i.e., OHCs elongate and contract in response to acoustical stimulation. This motility is made possible by both the cytoskeleton beneath the OHC plasma membrane and the motor protein prestin distributed throughout the plasma membrane. However, these factors have not yet been fully clarified. In the present study, therefore, attempts were made to observe the ultrastructure of the cytoskeleton of Guinea pig OHCs and to identify the motor protein prestin expressed in the plasma membrane of Chinese hamster ovary (CHO) cells by atomic force microscopy (AFM). Results indicate that the OHC cytoskeleton is comprised of circumferential actin filaments and spectrin cross-links and that particle-like structures with a diameter of 8–12 nm which exist in the plasma membrane of the prestin-expressing CHO cells are most likely be prestin.
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M3 - Conference contribution
AN - SCOPUS:85087096806
T3 - Auditory Mechanisms: Processes and Models - Proceedings of the 9th International Symposium
SP - 26
EP - 33
BT - Auditory Mechanisms
A2 - Nuttall, Alfred L.
A2 - Ren, Tianying
A2 - Gillespie, Peter
A2 - Grosh, Karl
A2 - de Boer, Egbert
PB - World Scientific Publishing Co. Pte Ltd
T2 - 9th International Mechanics of Hearing Workshop on Auditory Mechanisms: Processes and Models, MoH 2005
Y2 - 23 July 2005 through 28 July 2005
ER -