TY - JOUR
T1 - ATP-independent strand transfer protein from murine spermatocytes, spermatids, and spermatozoa
AU - Higashitani, Atsushi
AU - Tabata, Satoshi
AU - Ogawa, Tomoko
AU - Ogawa, Hideyuki
AU - Shibata, Masao
AU - Hotta, Yasuo
N1 - Funding Information:
This research has been supported by grants from the Ministry of Education, Science and Culture of Japan (62440090, 01790601, and 01615001), the National Institute of Health, U.S.A. (HD22504), the National Science Foundation, U.S.A. (DCB85-10556), and the American Cancer Society (CD-156C). We thank Dr. T. Shibata for supplying the monoclonal antibodies for recA (ARM191, ARM193, ARM321, ARM414) and Dr. H. Stern for critical discussion and help in the preparation the manuscript.
PY - 1990/2
Y1 - 1990/2
N2 - A protein-catalyzing D-loop formation is present in marine spermatocytes, spermatids, and spermatozoa, but is not found in somatic tissue or in premeiotic cells of the germline. Unlike the Escherichia coli RecA protein and the meiotic rec protein (m-rec) previously described, D-loop formation by this protein (referred to as "mAi-rec") does not require ATP. The meiotic profile of mAi-rec activity is only partly similar to that of m-rec. Like m-rec, it rises steeply during early prophase and reaches a peak at pachytene. Unlike m-rec, its activity remains high during the postmeiotic phase of spermatid development and is prominent in immunochemically stained spermatozoa. A polyclonal antibody to E. coli RecA reacts with mAi-rec and inhibits its activity. No such reaction occurs with m-rec protein. The extent of sequence homology between E. coli RecA and murine mAi-rec is highly limited; none of the several monoclonal antibodies tested reacted with mAi-rec.
AB - A protein-catalyzing D-loop formation is present in marine spermatocytes, spermatids, and spermatozoa, but is not found in somatic tissue or in premeiotic cells of the germline. Unlike the Escherichia coli RecA protein and the meiotic rec protein (m-rec) previously described, D-loop formation by this protein (referred to as "mAi-rec") does not require ATP. The meiotic profile of mAi-rec activity is only partly similar to that of m-rec. Like m-rec, it rises steeply during early prophase and reaches a peak at pachytene. Unlike m-rec, its activity remains high during the postmeiotic phase of spermatid development and is prominent in immunochemically stained spermatozoa. A polyclonal antibody to E. coli RecA reacts with mAi-rec and inhibits its activity. No such reaction occurs with m-rec protein. The extent of sequence homology between E. coli RecA and murine mAi-rec is highly limited; none of the several monoclonal antibodies tested reacted with mAi-rec.
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U2 - 10.1016/0014-4827(90)90311-W
DO - 10.1016/0014-4827(90)90311-W
M3 - Article
C2 - 2404773
AN - SCOPUS:0025062272
SN - 0014-4827
VL - 186
SP - 317
EP - 323
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -
