TY - JOUR
T1 - Augmentation of T H-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signaling
AU - Shimosato, Takeshi
AU - Kitazawa, Haruki
AU - Katoh, Shinichiro
AU - Tohno, Masanori
AU - Iliev, Iliyan Dimitrov
AU - Nagasawa, Chiho
AU - Kimura, Toshiro
AU - Kawai, Yasushi
AU - Saito, Tadao
N1 - Funding Information:
We sincerely thank Dr. J. Miyazaki (Division of Stem Cell Regulation Research, Osaka University Graduate School of Medicine) and Dr. H. Inadera (Department of Public Health, Toyama Medical and Pharmaceutical University) for their kind gifts of pCXN2.1-FLAG expression vector and pGLM-ENH-luci vector, respectively. This study was partly supported by a Grant-in-Aid for Scientific Research (B)(2) (No. 15380183) from the Japan Society for the Promotion of Science (JSPS) and the Skylark Food Science Institute to Dr. H. Kitazawa.
PY - 2005/1/28
Y1 - 2005/1/28
N2 - Toll-like receptor 9, which is expressed on the surface of antigen presenting cells and which was recently identified in the cytoplasmic follicle, recognizes bacterial CpG oligodeoxynucleotides (ODNs), resulting in the induction of a potent immune response. However, in our previous study, we found that TLR9 potentially recognizes not only CpG ODN but also non-CpG ODN such as AT ODN. Therefore, in the present study, to investigate this possibility, we elucidated the effects of AT ODN on T H-1, T H-2 type cytokine induction via TLR9 by real-time quantitative PCR analysis and ELISA of the swine TLR9 transfectant. The results demonstrated that the T H-1 type cytokines such as interleukin (IL)-12p70 and interferon (IFN)-γ were strongly induced by AT ODN compared to the unexposed controls, while T H-2 type cytokines were not induced. These results indicate that the AT ODN can augment the T H-1 immune response, which plays an important role in prevention of allergic responses. Moreover, the swine TLR9 transfectant demonstrated its usefulness for evaluation of immunostimulation by bacterial DNA through the detection of T H-1, T H-2 type cytokine induction via TLR9 signaling.
AB - Toll-like receptor 9, which is expressed on the surface of antigen presenting cells and which was recently identified in the cytoplasmic follicle, recognizes bacterial CpG oligodeoxynucleotides (ODNs), resulting in the induction of a potent immune response. However, in our previous study, we found that TLR9 potentially recognizes not only CpG ODN but also non-CpG ODN such as AT ODN. Therefore, in the present study, to investigate this possibility, we elucidated the effects of AT ODN on T H-1, T H-2 type cytokine induction via TLR9 by real-time quantitative PCR analysis and ELISA of the swine TLR9 transfectant. The results demonstrated that the T H-1 type cytokines such as interleukin (IL)-12p70 and interferon (IFN)-γ were strongly induced by AT ODN compared to the unexposed controls, while T H-2 type cytokines were not induced. These results indicate that the AT ODN can augment the T H-1 immune response, which plays an important role in prevention of allergic responses. Moreover, the swine TLR9 transfectant demonstrated its usefulness for evaluation of immunostimulation by bacterial DNA through the detection of T H-1, T H-2 type cytokine induction via TLR9 signaling.
KW - AT ODN
KW - Swine
KW - Toll-like receptor 9
KW - Transfectant
UR - http://www.scopus.com/inward/record.url?scp=10644272688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10644272688&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.11.119
DO - 10.1016/j.bbrc.2004.11.119
M3 - Article
C2 - 15607737
AN - SCOPUS:10644272688
SN - 0006-291X
VL - 326
SP - 782
EP - 787
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -