Automated preparation of hypoxic cell marker [18F]FRP-170 by on-column hydrolysis

Y. Ishikawa; Ren Iwata; S. Furumoto; Y. Takai

doi:10.1016/j.apradiso.2004.09.008

Automated preparation of hypoxic cell marker [¹⁸F]FRP-170 by on-column hydrolysis

Y. Ishikawa, Ren Iwata, S. Furumoto, Y. Takai

Cyclotron and Radioisotope Center (CYRIC)

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

An automated synthesis for the preparation of the novel hypoxic cell marker, [¹⁸F]FRP-170 3, [¹⁸F]1-[2-fluoro-1-(hydroxymethyl) ethoxy]methyl-2- nitroimidazole, was developed using an on-column basic-hydrolysis step. The ¹⁸F-labeled protected intermediate 2 was retained on a Sep-Pak Plus C18 cartridge and, in the same cartridge at room temperature, hydrolyzed by NaOH for deacetylation to give [¹⁸F]FRP- 170. The elution method from the cartridge was optimized for direct injection of the crude product into an HPLC column. Thus, [¹⁸F]FRP-170 was prepared in 20-30% decay-corrected radiochemical yield within 60 min.

Original language	English
Pages (from-to)	705-710
Number of pages	6
Journal	Applied Radiation and Isotopes
Volume	62
Issue number	5
DOIs	https://doi.org/10.1016/j.apradiso.2004.09.008
Publication status	Published - 2005 May 1

Keywords

Automated synthesis
F-18
FRP-170
Fluoromisonidazole
Hypoxic cell marker
On-column
Solid phase extraction

ASJC Scopus subject areas

Radiation

Access to Document

10.1016/j.apradiso.2004.09.008

Cite this

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title = "Automated preparation of hypoxic cell marker [18F]FRP-170 by on-column hydrolysis",

abstract = "An automated synthesis for the preparation of the novel hypoxic cell marker, [18F]FRP-170 3, [18F]1-[2-fluoro-1-(hydroxymethyl) ethoxy]methyl-2- nitroimidazole, was developed using an on-column basic-hydrolysis step. The 18F-labeled protected intermediate 2 was retained on a Sep-Pak Plus C18 cartridge and, in the same cartridge at room temperature, hydrolyzed by NaOH for deacetylation to give [18F]FRP- 170. The elution method from the cartridge was optimized for direct injection of the crude product into an HPLC column. Thus, [18F]FRP-170 was prepared in 20-30% decay-corrected radiochemical yield within 60 min.",

keywords = "Automated synthesis, F-18, FRP-170, Fluoromisonidazole, Hypoxic cell marker, On-column, Solid phase extraction",

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AU - Ishikawa, Y.

AU - Iwata, Ren

AU - Furumoto, S.

AU - Takai, Y.

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AB - An automated synthesis for the preparation of the novel hypoxic cell marker, [18F]FRP-170 3, [18F]1-[2-fluoro-1-(hydroxymethyl) ethoxy]methyl-2- nitroimidazole, was developed using an on-column basic-hydrolysis step. The 18F-labeled protected intermediate 2 was retained on a Sep-Pak Plus C18 cartridge and, in the same cartridge at room temperature, hydrolyzed by NaOH for deacetylation to give [18F]FRP- 170. The elution method from the cartridge was optimized for direct injection of the crude product into an HPLC column. Thus, [18F]FRP-170 was prepared in 20-30% decay-corrected radiochemical yield within 60 min.

KW - Automated synthesis

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KW - Hypoxic cell marker

KW - On-column

KW - Solid phase extraction

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